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I was chatting with another member and we believe it may have came from the substrate. I did a quick search and seen other people posting about this particular substrate and "instant-cycles" when using it. Not sure I believe all the hype per se, but cannot deny the results either (when using the seeding method).Gvilleguy said:Unless there was ammonia in your source water, not sure why that showed up initially. But the tank looks great! Nice job.