I think I may have found the problem, the above are all symptoms of CO2 poisoning, my test kit has now been proved to be at fault which means my CO2 levels have been at approx 75ppm for ages. How I really found out was when I had a slight cut on my finger and was cleaning the tank, every time my cut would come into contact it felt like I got a large electric shock!! Test kit from LFS confirms massive problems, no wonder I've been losing fish!
Large water change, aireation over night and a new kit / reduced CO2 - very happy fishes, now I can ensure that fishies are kept this way!