The 4dKH refers to the hardness of the water. You can't replace this with RO water unless your RO water happens to be exactly 4dKH as well.
The reason is that hardness affects how much CO2 can dissolve in the water which in turn affects the colour of your indicator. The 4dKH is just a calibration standard to ensure that your indicator is showing blue if too little, green just right and yellow too much CO2. Any other hardness of water than 4dKH would skew the results so that you wouldn't be sure what the colours meant anymore.