IMHO, if you are going to do a water change during a fishless cycle, there is no reason to do one less than 100%. If you are looking to clear nitrite, nitrate, increase the buffering capacity of your water again, whatever the reason, the more water you change the better the effect and the less water you really have to change in the long run. Yes another water change would be in order. Do 100% this time and nitrates will be at almost zero, so will the nitrites and your buffering capacity will be back to where it should be. As long as your filter media remains wet during this process (no reason it should dry out), your cycle will basically be unaffected. There might be a bit of a pause when you refill for a day or so, but ultimately, it will be better off in the end.
Go for it, The beauty of a fishless cycle is that you get to practice things like this (an emergency large volume water change) without any actual pressure or fish to get in the way. Pretend that this is a crisis management water change and see how your method works. Imagine how low you
could go with fish still being able to swim upright. Then, try to refill the tank with temp matched water to not shock the "fish" in the tank, as you would in a real crisis. No sense in
just doing a water change. Make it a drill and you will feel like this is an even greater benefit than just getting your cycle going better. Ultimately, you don't want your first crisis water change to be the first time you've add to do one. THat will be a little more stressful than it needs to be (or should be). Try one as a practice run and you will be better prepared for when a real one comes along.
