All tanks have co2 you don't have to add it. What the fish breath out is co2 there is co2 in tap water. So yes your tank had co2. It all depends on how much light you have. If you run high light the plants use up co2 faster and more of it then a plant in a low light or mid light tank.
If you were to not do any water changes and did not keep any fish no matter what you did your plants would die once they used up all the co2. It's like o2 for fish and us.
Yes if you don't clean your gravel and after lots of poo and food get in it that helps grow the plants and acts as a dirt for roots. But with sand poo will not get down to the root.
As mentioned by someone else there is a constant equilibrium with the atmospheric CO2 and the water column CO2.
You are also forgetting the fact that plants are living and therefore respire too (or "breathe" if you like).
C6H12O6 + CO2 => O2 + H2O + CO2
Even from decaying plant matter, bacteria will also be respiring!!
There is no way around it, it is impossible to achieve 0ppm CO2.
I also have to ask was you dosing any nutrients in the water column?
If you was only using root tabs then there is no way you can be sure that 100% of these root tabs remained in the substrate, therefore you are posting invalid information.
Good light, co2, and plant substrate is what you need for plants.
Not really, you cant use the word 'good', what does that mean?
A balance between light, CO2 & nutrients is what is needed for healthy plants growth.
OR
Unlimiting light, CO2 & nutrients is what is needed for maximum rate of plant growth.
substrate has nothing to do with it... explain rhizome type plants
The reason people see an increase in the growth rate is due to the fact you are providing extra nutrients! Try root tabs with EI, I bet you don't see a difference then because the nutrients are already unlimiting
aquatic plants much prefer to uptake the nutrients via the leaf as they use less energy doing so.
Where have you read that?
There have been experiments showing it depends entirely on the plant, with some prefering uptake through the roots (aka the "heavy root feeders") and some prefer leaf uptake (stem plants mainly) - This of course does not mean they won't use both sources.
It has also been found that root uptake is much quicker than leaf uptake.
Plants have to spend a lot of energy to obtain nutrients due to the concentration gradient, if anything I would imagine the nutrient concentration to be higher in the leaf than the roots, meaning it will be harder to absorb nutrients? That would link in with the fact that root uptake is quicker...
Thanks, Aaron