Wow, for someone that wants help, you'd think he didn't need it!
Bacteria under ideal conditions can double in number in approx. 30 minutes (generalization for aerobic bacteria in the lab). If from over feeding plus the guppy that was present, a colony of bacteria was established that could handle 2 guppies, upon adding the extra 2 fish over the load limit, over the course of say, 12 hours (a guesstimate, since I haven't worked with these species in the lab), the nitrifying bacteria could multiply and eliminate the extra ammonia and nitrite. However, before the bacteria can fully catch up to the new bioload, there is the potential for a small ammonia spike, which could push the female over the edge when added to the stress of moving her. By the time you notice the fish is dead and test the water, the conditions that caused the stress may have been eliminated. It takes the bacteria longer than 30min to adjust to the doubled bioload because the conditions are less than ideal (about 85F, highly oxygenated, and very high ammonia concentrations would be perfect, so in the aquarium it takes longer).
Source, a nearly completed (1 quarter to go) bachelor's degree in Microbiology/Microbial Genetics with a Biochemistry minor.
Bacteria under ideal conditions can double in number in approx. 30 minutes (generalization for aerobic bacteria in the lab). If from over feeding plus the guppy that was present, a colony of bacteria was established that could handle 2 guppies, upon adding the extra 2 fish over the load limit, over the course of say, 12 hours (a guesstimate, since I haven't worked with these species in the lab), the nitrifying bacteria could multiply and eliminate the extra ammonia and nitrite. However, before the bacteria can fully catch up to the new bioload, there is the potential for a small ammonia spike, which could push the female over the edge when added to the stress of moving her. By the time you notice the fish is dead and test the water, the conditions that caused the stress may have been eliminated. It takes the bacteria longer than 30min to adjust to the doubled bioload because the conditions are less than ideal (about 85F, highly oxygenated, and very high ammonia concentrations would be perfect, so in the aquarium it takes longer).
Source, a nearly completed (1 quarter to go) bachelor's degree in Microbiology/Microbial Genetics with a Biochemistry minor.