Cyanobacteria Questions & White Spores

Been reading this with interest, what has suprised me is the 'glucagon' element that was mentioned. I'm gonna do
a bit of digging and research about that, as glucagon is normally related sugar (normally stored in a persons liver!), now personally I don't think it will have anything to do with iron test kits as as we all know iron is a mineral and not a sugar. Unless it binds iron in some way. It will be interesting to know though, if not just for work reasons lol.
 
So, what I am taking home for me from this interesting thread is basically that if I have hard water (which I have), I should not be using Seachem flourish (for the trace elements)? If so, what should I use? Here in Mexico we have the choice between Seachem and Sera...
 
I have hard water Lu, and i have used Flourish for a couple of years now, with no problems. I'm sure that Mark and Andy mean the Iron test kits. Even though hard water contains trace elements, in a heavy plant tank, you'll still need to top up. Remember it's very hard to OD elements in a tank.

As for the Glucagon, where did this come from, i can't find it anywhere?

It appears glucagon will help in the uptake of Fe, you cannot get Iron glucagon though. Iron is in the forms of sulphate and gluconate, both again are common in my line of work, as glucagon is related to diabetes. As for the sulphates and gluconates, in humans we use the sulphates for any old tom, richard or harry, the glucontes are for people with poorer renal functions. The doses of gluconates are always higher.
 
I have hard water Lu, and i have used Flourish for a couple of years now, with no problems. I'm sure that Mark and Andy mean the Iron test kits. Even though hard water contains trace elements, in a heavy plant tank, you'll still need to top up. Remember it's very hard to OD elements in a tank.

Ok, thanks Ian! Happy about that as I still have another bottle of Flourish on the shelf...
 
I'm not talking test kits Ian. We are talking 'sequesters and chelators.

Not sure where Glucagon came from. I'm talking gluconate. the same as in iron pills for humans. The gluconate is a weak 'bonding' agent. That means that the iron is availble for a horter period and Seachem 'argue' that means it is better for the plants. Never been proven though and there isn't a high percentage of areas in the whole world with KH in the 1-3 range.

They fail to address the issue in that as KH rises it is available for shorter and shorter times. Much like CO2 what the plants don't use is lost to unavailable forms.

Different to CO2 within the substrate it can return to usable form where roots will access it however the moment it returns to the water column it oxidises and becomes unavailable again. lol

That is why we don't just put iron filings into the tank.

Seachem iron and flourish use gluconate. That is not to say it is not any good. Just that it is not going to be available to the plants for as long as something like TPN which uses EDTA (weak chelator) and HEEDTA (Strong chelator) More like a drip feed there.

The higher the KH you have (we are talking teens) the more useful the real strong chelators are as they hold the iron in usable form longer and the less useful the weaker ones are as they are lost much quicker.

So in essence it may look like we are saying if you have hard water then Seachem is useless to you however that is not the case. Iron is iron. Waht we are saying is that in a hard water tank you may be 'wasting' a lot when if you were using a stronger chelator in the mix like DTPA then you will waste less and may achieve better results. They will get iron from the Seachem. It is more a case of how long it will be available to the plants and how much they can get.

The reason I spoke about test kits and led into this chelation debate is because it is common to see a 1ppm dose disappear by the end of the day. This does not mean the plants have used up 1ppm. It is more likely tht the iron is there but now in a form the test kit cannot determine.

It is also common to see a reading and think things are fine when in fact the reading for iron is reading unavailable iron. Therefore the reading says positive when the actual is defficiency.

Different tests yield differing results via differing methods of measuring. They are both right because they tell you exactly what they are measuring for. It is a case of are they measuring for what you want to know? Yet another reason why we don't test with hobby test kits, especially with metals that are chelated and also phosphates. Thes kits are the most difficult to get 'real' readings from.

At the price the powders are for a couple of years supply (curca £2/$3) then its a no brainere in my eyes to spend that amount or more on Seachem Flourish to last 6 months.

This thread may be of more use to you. When people in this thread mention TMG they are talking about TPN (Not TPN+) It used to be called Tropica Master Grow and a lot of folks still call it that:
http://www.barrreport.com/showthread.php/2768-Chelated-vs-non-chelated-iron


AC
 
So I understood it right... I don't want to hijack this thread, but I have just sent a message to Sera asking them which chelator they are using in their Florena product (minerals and trace).

Alternatively, how could I mix my own trace? Remember, I cannot get things imported (it will get lost or stolen in the mail), so buying off the net is not an option for me.

EDIT: I understand that buying iron pills for humans would be the same as continuing with Seachem, right?
 
So I understood it right... I don't want to hijack this thread, but I have just sent a message to Sera asking them which chelator they are using in their Florena product (minerals and trace).

Alternatively, how could I mix my own trace? Remember, I cannot get things imported (it will get lost or stolen in the mail), so buying off the net is not an option for me.

EDIT: I understand that buying iron pills for humans would be the same as continuing with Seachem, right?

plus more likely than not they will have 'added extras in like other vitamins.

If things are going well with the Seachem I wouldn't worry. What we are talking about here is highlight and higher uptake so it may well be that with less light and slower uptakes the Seachem is providing all that is needed.

Not sure how to help you on the powders. Maybe one of those scpaers from Mexico(There must be 10 or 12 in that guys club) could help you. they may even be doing exactly as you and using Seachem ;)

AC
 
GH is pretty useless really. Just tells you how much Calcium/Magnesium is in the water. KH is the one/ is a little less than mine so I would guess your water is medium to hard? No idea. Not something I worry about to research in depth.

TH if you want to worry too much about iron and different chelators etc you should be getting your dry powders, mixing your own ferts and seeing for yourself what results you get. There are EDTA/DTPA powder mixes on ebay for under £2 which will suffice most people for a couple of years.

I don't really have the time to be mixing powders, it sounds like a messy job which requires certain ratios of substance. Are there any EDTA and DTPA chelating agents out there already premixed into a fert product? As you mentioned earlier in the post, EasyLife are secretive about what they include in their products so maybe I should invest in fertilisers from a more open and honest company. I'd be willing to give TPN+ another go but I'd really like to know if there is another highly respected product out there which contains a chelate which will last longer in the water column.

I have some bad news to tell regarding my aquarium. The cyanobacteria is back; I've had to shake every single plant in the aquarium to remove sheets of it contained on every single root and leaf. Tonnes of it went down the sink. I've now blacked out the aquarium again, and intend to keep it that way until Saturday afternoon, not only because I'm getting sick to death of the problem, but, yes, it will hopefully kill off the cyanobacteria as well.

I'm still confused as to what is causing it to thrive, I've established the following as being possible causes:

1. Lack of flow (and the lack of 02 that comes with it) - I've installed a Hydor Koralia Nano 900 powerhead, but cyanobacteria is still growing.
2. Overabundance of nutrients - phosphate 1.0 - 1.5 ppm, Nitrate 20 ppm, Iron 0.2 ppm (probably not very representative of the actual concentration) + other nutrients.
3. Dirty substrate - It appears clean, there is no 'smoke effect' when I apply the gravel vac to my substrate which usually is an indicator that it's dirty. Despite being clean, cyanobacteria is growing on the sand particles.
4. Lack of Nitrate - I increased the concentration to 20ppm. Still not enough?
5. "Plant growth problem" - A person named 'plantbrain' in this thread states plant growth problems are the main cause of algae problems. Why then do I have cyanobacteria when 75% of my plants are growing well.

I've seen many planted aquarium videos online and it became apparent that their plants do not contain algae, nor does the glass or substrate. There must be something fundamentally wrong with either my filter system (not the best in the world it would seem) or I must not be good at knowing how much nutrients my plants need. I've found that when it comes to nutrient-dosing, it seems like a guessing game; should it be?


I'll list all of my aquarium details in the hope that somebody can tell me what they would do or what they would do differently if they owned this particular tank in terms of providing plants their required nutrients, in the correct chelated form and in a way which will not encourage any form of algae growth.

Tank length: 60cm
Tank capacity: About 110 Litres.
Plants: 5-6 egeria densa strands, 3 very large x limnophila sessiliflora, 1 large crytocoryne undulata broad leaves, 2 x small off-shoots of the cryptocoryne, 7 x strands of bacopa monnieri, 1 x lilaeopsis brasiliensis.
Stock: 1 German Blue Ram, 5 Black Neon Tetra, 2 Dwarf suckermouths, 1 Dwarf Neon Rainbowfish.
C02 unit: Dennerle Comfort-Line CO2 Professional
Lighting: 2 x 24w T5 strip bulbs
Filter system: Cayman 05 internal filter.
Substrate: Caribsea Instant Sand - Torpedo Beach
Decor: Small pieces of bog wood.
Water parameters: Free ammonia 0 ppm, ammonium 1.0ppm, nitrite 0 ppm, nitrate 20 ppm. PH: 7.4, KH: 5dH, GH: 8dH, Iron: 0.2 ppm, Phosphate: 1.0 ppm.

Maintenance Regime: (feeding, nutrient dosing, cleaning etc)

-2-3 gravel cleans using motorised gravel vac per week.
-Daily dosing of 2 ml of Easycarbo
-Dosing of EasyLife nitro to keep nitrate level at 20ppm. I will usually apply 2.5ml if nitrate level goes low which it often doesn't
- Dosing of 2.5ml EasyLife Profito every 1-2 days. May also supplement with EasyLife Ferro too on the same days.
- Dosing of 5.0ml of Easylife Fosfo if Phosphate level goes below 1.0 ppm.
- 30-40% water change conducted every 4 or 5 days.
- Dennerle Comfort-Line CO2 Professional system set to release 1 bubble of c02 every 4 seconds.
- feeding of one quarter chunk of Tetra Tabimin once per day for cat fish. About 7-8 pieces of fish flake applied from the Ocean-nutrition product range 2-3 times a day for rest of fish.
 
I do not know whether it contributes to the problem or not, but why are you still dosing easycarbo if you have a CO2 set-up? That seems double to me. I dose easycarbo as I don't have any other source of CO2 in my tank, but you are using 2 sources now. I would quit the easycarbo, unless you want to spot dose to get rid of local algae...
 
I do not know whether it contributes to the problem or not, but why are you still dosing easycarbo if you have a CO2 set-up? That seems double to me. I dose easycarbo as I don't have any other source of CO2 in my tank, but you are using 2 sources now. I would quit the easycarbo, unless you want to spot dose to get rid of local algae...
Well I'm following the EasyLife instructions which apply for high-tech tanks (tanks with strong lighting).
 
Mmmm, but does easylife say you should have a CO2 system AND using liquid carbon? Why don't you stop using liquid carbon for a while and see if it makes any difference?
Yes it does, where the lighting is high-tech (i.e. T5 or T8 with high wattage).

I use the Easycarbo on and off. I tend to rely on it when my drop checker is showing a dark green colour which indicates a low c02 concentration. If I find the drop-checker has a green solution I don't use the Easycarbo.
 
Firstly I'll state there is no problem dosing Easycarbo on top of CO2 injection. As it is liquid C and not CO2 it will no make the CO2 levels unstable. They are 2 different things.

It is more of a waste issue and something that should be short term until the CO2 is sorted out. Easylife are selling product if they say you should be adding it as well as CO2 but it will not hurt. So it won't harm the tank but you shouldn't need to be adding both.

I don't really have the time to be mixing powders, it sounds like a messy job which requires certain ratios of substance.

Mixing dry ferts is quicker than going to the shop for a bottled product. Takes me all of 10 minutes and the bottles (1 macro and 1 trace) will last me longer than the bottled product (circa 2 months) making 1 hour a year. Not a case of Ratios, there are several component powders, then you can use either a 'herb/jewellery' scale or a proper measuring spoon(s) and make up your mix with DI water. Pretty simple stuff.

Are there any EDTA and DTPA chelating agents out there already premixed into a fert product? As you mentioned earlier in the post, EasyLife are secretive about what they include in their products so maybe I should invest in fertilisers from a more open and honest company. I'd be willing to give TPN+ another go but I'd really like to know if there is another highly respected product out there which contains a chelate which will last longer in the water column.

TPN/TPN+ and Seachem are the only ones I suggest. They are the only ones (in single bottles) that seem to understand the 'phosphates DO NOT cause algae' philosophy rather than pandering to myths. Easylife and others DO understand but supply all their products in several bottles which makes dosing more of a pain than TPN+ or dry ferts in solution.

I have some bad news to tell regarding my aquarium. The cyanobacteria is back; I've had to shake every single plant in the aquarium to remove sheets of it contained on every single root and leaf. Tonnes of it went down the sink. I've now blacked out the aquarium again, and intend to keep it that way until Saturday afternoon, not only because I'm getting sick to death of the problem, but, yes, it will hopefully kill off the cyanobacteria as well.

I said it would. You can kill it off but the 'encouraging' conditions need to be addressed to stop it from re-occurring.

1. Lack of flow (and the lack of 02 that comes with it) - I've installed a Hydor Koralia Nano 900 powerhead, but cyanobacteria is still growing.
Flow is important but it needs to be in conjunction with the nutrients being correct. Being able to distribute the goods well is great IF the goods are there to be distributed.

Overabundance of nutrients - phosphate 1.0 - 1.5 ppm, Nitrate 20 ppm, Iron 0.2 ppm (probably not very representative of the actual concentration) + other nutrients.
Forget the word 'overabundance' That is what one of the key problems is. You are way below what I dose. All of these can be increased without fear. 20ppm Nitrate should be OK however many will already have 20ppm in their tap water and still dose on top. The ranges above should however be enough for you if the readings are correct.

3. Dirty substrate - It appears clean, there is no 'smoke effect' when I apply the gravel vac to my substrate which usually is an indicator that it's dirty. Despite being clean, cyanobacteria is growing on the sand particles.
I have no idea on this one. I do not gravel vac at all. My water changes are performed by removing my external filter hoses and emptying water out that way. Substrate does not get touched.


4. Lack of Nitrate - I increased the concentration to 20ppm. Still not enough?
Possibility if the test kit is not giving correct readings.

5. "Plant growth problem" - A person named 'plantbrain' in this thread states plant growth problems are the main cause of algae problems. Why then do I have cyanobacteria when 75% of my plants are growing well.

'Plantbrain' is Tom Barr. He is the man you should listen to. The problem here is you are taking the statement on algae but applying it to a bacteria. Cyanobacteria IS NOT an algae.

I've seen many planted aquarium videos online and it became apparent that their plants do not contain algae, nor does the glass or substrate. There must be something fundamentally wrong with either my filter system (not the best in the world it would seem) or I must not be good at knowing how much nutrients my plants need. I've found that when it comes to nutrient-dosing, it seems like a guessing game; should it be?

One problem is that you see the 'ideal' in photos and videos. I may show you a picture of a perfect algae free tank and you say 'how does he do that'. You have no way of knowing if I spent an hour cleaning algae and Cyano etc prior to taking photos or videos. So whilst admiring the pictures and videos take their 'algae free' appearance with a pinch of salt. There are some who claim to have algae free tanks who are putting in hours of cleaning work :) (Not me or Plantbrain or Ianho)

Stick to the reality of getting it right behind the scenes. Much better to spend the time at the beginning, learn the system, get it right and then spend the rest of you life saying 'In the end it was quite simple'

That filter is not cutting the mustard. I would suggest it's capacity is half what it neeeds to be. Not even up to scratch in terms of turnover as the Fluval Internals. A 125ltr Fluval tank would come with a 700lph or higher internal filter.

The turnover not only means better flow but it also means it draws more of the dirty water through eavery hour and therefore removes more detritus. It will also keep more of the dirt from hitting the substrate. The filter MAY be the cause of many of your problems however I am hesitant to state this as fact as it may not be and also you may still have other isues which after changing filter you may think 'I have the problem still so it was not the filter'.

I'll list all of my aquarium details in the hope that somebody can tell me what they would do or what they would do differently if they owned this particular tank in terms of providing plants their required nutrients, in the correct chelated form and in a way which will not encourage any form of algae growth.

Forget the chelates. We have gotten off track in this thread. I started talking about Chelates when you were putting up Fe test readings to explain that the reading is not overly useful in that it could either show iron as present when it is not available or it can show iron as zero when in fact it is there but in unreadable form.

Tank capacity: About 110 Litres.
About? What volume did it say on the box? Go by that. If it said 125 litres then go by that and not try and figure out water volumes and hardscape displacements etc.

Filter system: Cayman 05 internal filter.
I would put this on ebay and replace either with a higher turnover/capacity internal like a Fluval 3+/4+ or an external filter with a turnover of 700lph or more.

The water parameters are fine but I wonder about the Ph. Is 7.4 with CO2 addition? Does that mean your tap water is circa 8 or does it mean that the CO2 is not working very well?

WOW you do a lot of work on this tank. I do 1 x 50% water change a week with no gravel vac. My filter and pipes are cleaned once a month and I dose from 2 bottles daily. It doesn't need to be so hard to have 'algae free' tanks with 'gin' clear water.

Dennerle Comfort-Line CO2 Professional system set to release 1 bubble of c02 every 4 seconds
This is very low for your size tank and lighting hence why you are needing the Easycarbo. I would be in the 2-3 bubbes per second region if it were me and that would be with perfect flow and diffusion. I would increase this via the needle valve incrementally per day watching the drop checker until you get a stable light green at lights on every day. This may well account for the Ph reading being higher than I expected.

Be careful though and increase by small amounts daily to reassure yourself and to not risk gassing the fish.

Fish feeding isn't a problem. Overfeed, underfeed shouldn't be a 'real' problem. I overfeed a lot with no consequence other than more snails.

In essence I think you could do with more filtration if only to remove more detritus from the tank water. Will also boost flow too.

On Nutrients I think you are working very hard with all those bottles and testing etc. Life should be easy and dry ferts are just that. Similar to TPN+ where you just dose 1 bottle.

This game isn't about ratios and measuring and testing. That is for those who want to get into the science, dial into the exact needs and uptakes etc. Dose more than you need and then water change to remove some of the excess/ammonia. Continue that and you can forget about nutrient defficiency being the cause of anything and concentrate on other things like circulation and CO2 which are the cause of 95% of algae problems. A high percentage of those who dose excess still have these problems because they fail to get the CO2/circulation right. They often think it is good and blame excess nutrient or insufficient nutrient. They are in nearly all cases not getting the CO2 right for the 'system' they have.

Now a little re-assurance. If I post a picture or video it will look perfectly 'algae free'. In essence it is but there is no such thing as an 'algae free' tank. There is such a thing as a minimal algae tank.

So when you get close up to my tank and see what you can't see in photos or videos there are little bits of GSA on a few Anubias leaves (not all.) There are small amounts of BBA on wood etc. Very very minimal and not noticeable unless you look really hard but it is still there.

That is the goal. Not to eradicate algae completely because that will not happen. It is to make it unnoticeable. Minimise it.

AC
 
Brilliant post Andy and I would also echo his last statement mark regarding the minimal and low algae. We all have some if only in a tiny amount, in our tanks, I have a small patch of gda on one of my back walls. I did start getting wound up about this patch a while back, I started tinkering with things to make it better. In the end I have resided myself that other than buy another koralia, I'm not gonna rid myself of the patch. It's in a place out of veiw.

Again just to reiterate andy again, up the filtration and up
the dosing of ferts, up the C02, steadily.

I really want you to get this right Mark, so you can start to enjoy your tank.
 
Mixing dry ferts is quicker than going to the shop for a bottled product. Takes me all of 10 minutes and the bottles (1 macro and 1 trace) will last me longer than the bottled product (circa 2 months) making 1 hour a year. Not a case of Ratios, there are several component powders, then you can use either a 'herb/jewellery' scale or a proper measuring spoon(s) and make up your mix with DI water. Pretty simple stuff.

I don't know what a 'herb/jewellery' scale is. What exactly should I be looking for to be putting into the distilled water? I understand what macro means (short for macro-nutrient) but what is a trace?


TPN/TPN+ and Seachem are the only ones I suggest. They are the only ones (in single bottles) that seem to understand the 'phosphates DO NOT cause algae' philosophy rather than pandering to myths. Easylife and others DO understand but supply all their products in several bottles which makes dosing more of a pain than TPN+ or dry ferts in solution.

But you said the Seachem fert contains a short-lasting chelate which may not last long enough for the plant to utilise it. How many mls of TPN+ or Seachem would YOU be looking to dose in MY tank if you OWNED it?



I said it would. You can kill it off but the 'encouraging' conditions need to be addressed to stop it from re-occurring.

The 'encouraging conditions' are lack of flow and not enough nutrients from what I understand? The guessing-game I apply when trying to work out how much macro/micro nutrients I need to apply is the obvious cause of disproportionate level of available nutrients in relation to the plant's requirements. So, what exactly constitutes a proportionate level? Is the dosing of nutrients correct simply by noting that algae growth is more limited?


Possibility if the test kit is not giving correct readings.

It's the API branded test kit you know, lol. Don't get the impression that I'm using interpet's master test kit which is highly inaccurate and has been recalled once or twice.


'Plantbrain' is Tom Barr. He is the man you should listen to. The problem here is you are taking the statement on algae but applying it to a bacteria. Cyanobacteria IS NOT an algae.

Wikipedia seems to refer to cyanobacteria has part bacteria part algae, but the means of eradication are to be distinguished from the means of eradication of algae.


One problem is that you see the 'ideal' in photos and videos. I may show you a picture of a perfect algae free tank and you say 'how does he do that'. You have no way of knowing if I spent an hour cleaning algae and Cyano etc prior to taking photos or videos. So whilst admiring the pictures and videos take their 'algae free' appearance with a pinch of salt. There are some who claim to have algae free tanks who are putting in hours of cleaning work :) (Not me or Plantbrain or Ianho)

Ok, point taken there. I didn't think people were that tricky to be honest.


That filter is not cutting the mustard. I would suggest it's capacity is half what it neeeds to be. Not even up to scratch in terms of turnover as the Fluval Internals. A 125ltr Fluval tank would come with a 700lph or higher internal filter.

Would it be worth me putting a separate internal filter into the aquarium; preferably a smaller filter but with high performance in terms of the LPH rate? Not sure if this would cause a bit of issues for the beneficial bacteria in the other established filter.

The turnover not only means better flow but it also means it draws more of the dirty water through eavery hour and therefore removes more detritus. It will also keep more of the dirt from hitting the substrate. The filter MAY be the cause of many of your problems however I am hesitant to state this as fact as it may not be and also you may still have other isues which after changing filter you may think 'I have the problem still so it was not the filter'.

I've sat and watched the filter for several minutes and it would appear that the grills are taking in the smallest pieces of dirt. Other particles will go above or below the filter system. The substrate can get noticeably more dirty underneath or slightly in front of the filter system implying that there is a inward flow produced by the grills but it isn't sufficient to carry the particles into the filter so instead they land on the substrate until I come a long and get it out.


This is very low for your size tank and lighting hence why you are needing the Easycarbo. I would be in the 2-3 bubbes per second region if it were me and that would be with perfect flow and diffusion. I would increase this via the needle valve incrementally per day watching the drop checker until you get a stable light green at lights on every day. This may well account for the Ph reading being higher than I expected.

The PH is 7.4 out of the tap. The c02 system maintains it at 7.4, sometimes taking it down to 7.2. Without the c02 system it goes to 8.4 over a 4 day period.


This game isn't about ratios and measuring and testing. That is for those who want to get into the science, dial into the exact needs and uptakes etc. Dose more than you need and then water change to remove some of the excess/ammonia.

Hopefully you mean ammonium? :look:


So when you get close up to my tank and see what you can't see in photos or videos there are little bits of GSA on a few Anubias leaves (not all.) There are small amounts of BBA on wood etc. Very very minimal and not noticeable unless you look really hard but it is still there.

I think i've always expected there to be some algae present, but when I get algae it's horrific. It looks like gravy has been placed on all the plant leaves. Thats what I want to avoid and I'll see if I can eradicate it by addressing the flow issues.
 

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