How big are the clown loaches as the tanks to small for 8 of them.
What test kit are you using.
Signs of flukes are.
Opaque body with excess slime.
Red inflamed gills, or pale gills with excess slime.
Flicking and rubbing.
Darting, erratic swimming.
Laboured breathing, or gasping.
Sores on the body of the fish.
Weight loss.
Spitting food out.
Swimming in a jerky movement.
Where've you been hiding?
/www.waterlife.co.uk/waterlife/protozin.htm