I would expect evolution takes a lot longer than a couple of decades, however some research appears to indicate that inbreeding reduces the ability of guppies to handle salinity, though I would attribute that to the perils of inbreeding rather than any form of rapid evolution occuring.Personally i wouldn't put guppys in a marine tank, most guppys found in petshops come from long strains of guppys which have been bred and produced in freshwater conditions for decades, i'm sure all this breeding in freshwater would have made guppys lose some of their tolerance to marine conditions,
Females that stayed in 150% sea water (58.5%) for 30 days had embryos in their gonads.