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% Of Toxic Ammonia Charts

Discussion in 'Tropical Chit Chat' started by Bignose, Aug 18, 2006.

  1. Bignose

    Bignose Birds just don't know how to follow the rules.
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    Since the tests for our aquariums just read the total ammonia content in the water, the question has come up how much of that ammonia is actually toxic? This is a complicated question, which really has to do with the ammonia-ammonium equilibrium, ammonia being toxic, ammonium being comparatively much less toxic. Ammonium is not harmless, there is evidence it interferes with the normal salt exchange at the fish gills, but it is not anywhere as dangerous as ammonia.

    Also I'd just like to make it clear that any level of ammonia will cause health problems. Ammonia can cause gill damage, that could become permanent, and a very recent article ("Low levels of environmental ammonia increase susceptibility to disease in Chinook salmon smolts " by Ackerman PA, Wicks BJ, Iwama GK, Randall DJ in PHYSIOLOGICAL AND BIOCHEMICAL ZOOLOGY volume 79 JUL-AUG 2006) showed that fish exposed to low levels of ammonia were more susceptible to disease later on in life. But this mini-article is about when emergency action needs to be taken.

    The split between the two species (ammonia and ammonium) is determined by equilibrium. The chemistry reaction can be written as:

    NH3 + H+ <--> NH4+
    (ammonia) + (H+ ions) <--> (ammonium+ ions)

    The +'s indicating a charge of +1, and <--> indicating that the reaction can go both ways.

    pH is a measure of the concentration of H+ ions. pH really means -log(base 10) of H+. For example, pH of 7.0 means a concentration of 10^-7 H+ ions, a pH of 5.0 means a concentration of 10^-5 H+ ions.

    Therefore the lower the pH, the higher the concentration of H+ ions.

    Now, with more H+ ions present, the above equilibrium chemical reaction gets pushed towards the right hand side. This is known as Le Chatelier's Principle. But, Le Chatelier's is a general rule, it does not give us the exact splits.

    The calculate the splits, we need the equilibrium constant K = [NH3][H+]/[NH4+] where [] stands for the concentration of the species in the []'s. K is a function of temperature, so experimentation needed to be performed, and some high level computations (well beyond the scope of this mini-article) needed to be performed.

    Clegg, S. and Whitfield, M did that experiment. (Their paper is "A chemical model of seawater including dissolved ammonia and the stoichiometric dissociation constant of ammonia in estuarine water and seawater from -2 to 40 deg C. Geochimica et Cosmochimia Acta Volume 59, 1995.) They found the equilibrium constant as a function of temperature and salinity.

    So with this information, I calculated what % of the total ammonia in the water would be toxic ammonia and ammonium.

    How to use the following tables

    Know your pH and temperature.
    Look up the % toxic ammonia on the following charts for your temp and pH
    Know how much ammonia you have in your tank
    Multiply your ammonia content by the looked up percentage
    If this number is over 0.02 ppm, then immediate action needs to be taken.

    Example:
    I have a tank at 27 deg with pH 6.6 water and 2.0 ppm of ammonia.
    % toxic ammonia (from) the chart is: 0.045%

    2.0*(0.00045)=0.008 ppm toxic ammonia, so it is not immediately dangerous, though exposure to any ammonia is unhealthy for the fish.

    Note that if the pH of that tank was over 8.0, the level of ammonia would be immediately deadly, so pH does count!

    Also, these are only for freshwater, if there is enough demand I might be talked into making up the equivalent charts for saltwater tanks, but not now.

    20 deg C or 68.0 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 9.6835
    8.8 ... 6.3363
    8.6 ... 4.0937
    8.4 ... 2.6225
    8.2 ... 1.6709
    8.0 ... 1.0608
    7.8 ... 0.6719
    7.6 ... 0.4250
    7.4 ... 0.2686
    7.2 ... 0.1696
    7.0 ... 0.1071
    6.8 ... 0.0676
    6.6 ... 0.0427
    6.4 ... 0.0269
    6.2 ... 0.0170
    6.0 ... 0.0107
    5.0 ... 0.0011
    4.0 ... 0.0001


    21 deg C or 69.8 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 9.7474
    8.8 ... 6.3797
    8.6 ... 4.1224
    8.4 ... 2.6412
    8.2 ... 1.6829
    8.0 ... 1.0685
    7.8 ... 0.6768
    7.6 ... 0.4281
    7.4 ... 0.2706
    7.2 ... 0.1709
    7.0 ... 0.1079
    6.8 ... 0.0681
    6.6 ... 0.0430
    6.4 ... 0.0271
    6.2 ... 0.0171
    6.0 ... 0.0108
    5.0 ... 0.0011
    4.0 ... 0.0001


    22 deg C or 71.6 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 9.8113
    8.8 ... 6.4231
    8.6 ... 4.1511
    8.4 ... 2.6599
    8.2 ... 1.6949
    8.0 ... 1.0762
    7.8 ... 0.6817
    7.6 ... 0.4312
    7.4 ... 0.2725
    7.2 ... 0.1721
    7.0 ... 0.1087
    6.8 ... 0.0686
    6.6 ... 0.0433
    6.4 ... 0.0273
    6.2 ... 0.0172
    6.0 ... 0.0109
    5.0 ... 0.0011
    4.0 ... 0.0001


    23 deg C or 73.4 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 9.8751
    8.8 ... 6.4664
    8.6 ... 4.1798
    8.4 ... 2.6786
    8.2 ... 1.7069
    8.0 ... 1.0838
    7.8 ... 0.6866
    7.6 ... 0.4343
    7.4 ... 0.2745
    7.2 ... 0.1734
    7.0 ... 0.1095
    6.8 ... 0.0691
    6.6 ... 0.0436
    6.4 ... 0.0275
    6.2 ... 0.0174
    6.0 ... 0.0110
    5.0 ... 0.0011
    4.0 ... 0.0001


    24 deg C or 75.2 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 9.9388
    8.8 ... 6.5097
    8.6 ... 4.2085
    8.4 ... 2.6973
    8.2 ... 1.7190
    8.0 ... 1.0915
    7.8 ... 0.6915
    7.6 ... 0.4374
    7.4 ... 0.2764
    7.2 ... 0.1746
    7.0 ... 0.1102
    6.8 ... 0.0696
    6.6 ... 0.0439
    6.4 ... 0.0277
    6.2 ... 0.0175
    6.0 ... 0.0110
    5.0 ... 0.0011
    4.0 ... 0.0001



    25 deg C or 77.0 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.0025
    8.8 ... 6.5530
    8.6 ... 4.2372
    8.4 ... 2.7159
    8.2 ... 1.7310
    8.0 ... 1.0992
    7.8 ... 0.6964
    7.6 ... 0.4405
    7.4 ... 0.2784
    7.2 ... 0.1758
    7.0 ... 0.1110
    6.8 ... 0.0701
    6.6 ... 0.0442
    6.4 ... 0.0279
    6.2 ... 0.0176
    6.0 ... 0.0111
    5.0 ... 0.0011
    4.0 ... 0.0001


    26 deg C or 78.8 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.0661
    8.8 ... 6.5963
    8.6 ... 4.2658
    8.4 ... 2.7346
    8.2 ... 1.7430
    8.0 ... 1.1069
    7.8 ... 0.7013
    7.6 ... 0.4436
    7.4 ... 0.2804
    7.2 ... 0.1771
    7.0 ... 0.1118
    6.8 ... 0.0706
    6.6 ... 0.0445
    6.4 ... 0.0281
    6.2 ... 0.0177
    6.0 ... 0.0112
    5.0 ... 0.0011
    4.0 ... 0.0001


    27 deg C or 80.6 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.1296
    8.8 ... 6.6396
    8.6 ... 4.2945
    8.4 ... 2.7533
    8.2 ... 1.7550
    8.0 ... 1.1146
    7.8 ... 0.7062
    7.6 ... 0.4467
    7.4 ... 0.2823
    7.2 ... 0.1783
    7.0 ... 0.1126
    6.8 ... 0.0711
    6.6 ... 0.0449
    6.4 ... 0.0283
    6.2 ... 0.0179
    6.0 ... 0.0113
    5.0 ... 0.0011
    4.0 ... 0.0001


    28 deg C or 82.4 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.1931
    8.8 ... 6.6828
    8.6 ... 4.3232
    8.4 ... 2.7720
    8.2 ... 1.7671
    8.0 ... 1.1223
    7.8 ... 0.7110
    7.6 ... 0.4498
    7.4 ... 0.2843
    7.2 ... 0.1796
    7.0 ... 0.1134
    6.8 ... 0.0716
    6.6 ... 0.0452
    6.4 ... 0.0285
    6.2 ... 0.0180
    6.0 ... 0.0113
    5.0 ... 0.0011
    4.0 ... 0.0001


    29 deg C or 84.2 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.2565
    8.8 ... 6.7260
    8.6 ... 4.3518
    8.4 ... 2.7906
    8.2 ... 1.7791
    8.0 ... 1.1300
    7.8 ... 0.7159
    7.6 ... 0.4529
    7.4 ... 0.2863
    7.2 ... 0.1808
    7.0 ... 0.1142
    6.8 ... 0.0721
    6.6 ... 0.0455
    6.4 ... 0.0287
    6.2 ... 0.0181
    6.0 ... 0.0114
    5.0 ... 0.0011
    4.0 ... 0.0001


    30 deg C or 86.0 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.3198
    8.8 ... 6.7692
    8.6 ... 4.3805
    8.4 ... 2.8093
    8.2 ... 1.7911
    8.0 ... 1.1376
    7.8 ... 0.7208
    7.6 ... 0.4560
    7.4 ... 0.2882
    7.2 ... 0.1820
    7.0 ... 0.1149
    6.8 ... 0.0726
    6.6 ... 0.0458
    6.4 ... 0.0289
    6.2 ... 0.0182
    6.0 ... 0.0115
    5.0 ... 0.0012
    4.0 ... 0.0001


    31 deg C or 87.8 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.3831
    8.8 ... 6.8123
    8.6 ... 4.4091
    8.4 ... 2.8280
    8.2 ... 1.8032
    8.0 ... 1.1453
    7.8 ... 0.7257
    7.6 ... 0.4591
    7.4 ... 0.2902
    7.2 ... 0.1833
    7.0 ... 0.1157
    6.8 ... 0.0730
    6.6 ... 0.0461
    6.4 ... 0.0291
    6.2 ... 0.0184
    6.0 ... 0.0116
    5.0 ... 0.0012
    4.0 ... 0.0001


    32 deg C or 89.6 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.4463
    8.8 ... 6.8554
    8.6 ... 4.4378
    8.4 ... 2.8467
    8.2 ... 1.8152
    8.0 ... 1.1530
    7.8 ... 0.7306
    7.6 ... 0.4622
    7.4 ... 0.2922
    7.2 ... 0.1845
    7.0 ... 0.1165
    6.8 ... 0.0735
    6.6 ... 0.0464
    6.4 ... 0.0293
    6.2 ... 0.0185
    6.0 ... 0.0117
    5.0 ... 0.0012
    4.0 ... 0.0001


    33 deg C or 91.4 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.5094
    8.8 ... 6.8985
    8.6 ... 4.4664
    8.4 ... 2.8653
    8.2 ... 1.8272
    8.0 ... 1.1607
    7.8 ... 0.7355
    7.6 ... 0.4653
    7.4 ... 0.2941
    7.2 ... 0.1858
    7.0 ... 0.1173
    6.8 ... 0.0740
    6.6 ... 0.0467
    6.4 ... 0.0295
    6.2 ... 0.0186
    6.0 ... 0.0117
    5.0 ... 0.0012
    4.0 ... 0.0001


    34 deg C or 93.2 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.5724
    8.8 ... 6.9416
    8.6 ... 4.4950
    8.4 ... 2.8840
    8.2 ... 1.8392
    8.0 ... 1.1684
    7.8 ... 0.7404
    7.6 ... 0.4684
    7.4 ... 0.2961
    7.2 ... 0.1870
    7.0 ... 0.1181
    6.8 ... 0.0745
    6.6 ... 0.0470
    6.4 ... 0.0297
    6.2 ... 0.0187
    6.0 ... 0.0118
    5.0 ... 0.0012
    4.0 ... 0.0001


    35 deg C or 95.0 deg F
    =============
    pH ... % toxic ammonia
    =============
    9.0 ... 10.6353
    8.8 ... 6.9846
    8.6 ... 4.5236
    8.4 ... 2.9026
    8.2 ... 1.8513
    8.0 ... 1.1761
    7.8 ... 0.7453
    7.6 ... 0.4716
    7.4 ... 0.2980
    7.2 ... 0.1883
    7.0 ... 0.1189
    6.8 ... 0.0750
    6.6 ... 0.0474
    6.4 ... 0.0299
    6.2 ... 0.0189
    6.0 ... 0.0119
    5.0 ... 0.0012
    4.0 ... 0.0001
     
  2. Bullrock74

    Bullrock74 Member

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    This is a very interesting article. I'm not joking, I'm being sincere.
     
  3. Lynden

    Lynden a "fish hater"

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    How long did it take you to write this?

    But, no matter. Truly, it was a good article. :clap:

    -Lynden
     
  4. Bignose

    Bignose Birds just don't know how to follow the rules.
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    10 mins to write the text, 10 mins in Excel (I didn't do all that by hand), 10 mins to format the charts so that they would display correctly on the forums. I actually just expanded on the work I did for your ammonia thread the other day, so a lot of it was already done.
     
  5. Lynden

    Lynden a "fish hater"

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    Good work. Maybe somebody will pin it.
     
  6. Arimus

    Arimus Member

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    If anyone wants it I've converted bignose's table back into Excel and added a lookup function - enter the temp in c, the ph and nh3/4 combined reading and it gives the % and nh3 values.

    PM with an email addy as I can't attach it and I'll send it.
     
  7. darryl864

    darryl864 Member

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    I can see that from your charts that if you have a high PH and a High temperature the same amount of Ammonia is more toxic to your fish if you had a low PH and a low temperature. In conclusion than people with high temps an PH should be more wary of their ammonia levels. I feel that it would be useful if something could be added to the calculator page below using your findings?

    http://www.fishforums.net/aquarium-calculator.htm

    Darryl
     
  8. waterdrop

    waterdrop Enthusiastic "Re-Beginner"

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    Bignose,

    I have read that 0.05 is the toxic threshold line for numbers coming out of a temp/pH calculated toxicity chart like that. You have used 0.02 and I wonder if any choice of number in this range is subjective, I guess it would be to some extent.

    Congrats on a nice clear explanation that covers all the details nicely!

    ~~waterdrop~~
     
  9. JacksonvilleKoiSupply

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    I am very interested in how you cam about your formula for these calculations. This link is for an online calculator that also tells you the amount of toxic ammonia in the water and the numbers in your charts are way off compared to the calculator. I don't know which one of you is correct but someone is...

    http://cnykoi.com/calculators/calcnh3c.asp

    Hopefully you can clarify what is going on here.


    PS. I know this is my first post here...I am not trying to stir any pot, or bad mouth anyone in any way. I am just looking for the correct way to find the total amount of toxic ammonia in the water.

    Thanks,
    Zac
     
  10. Bignose

    Bignose Birds just don't know how to follow the rules.
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    I referenced the article I got my formula from. "A chemical model of seawater including dissolved ammonia and the stoichiometric dissociation constant of ammonia in estuarine water and seawater from -2 to 40 deg C. Geochimica et Cosmochimia Acta Volume 59, 1995. You are free to read it and see what you think about it for yourself.
     

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