Nutrafin Cycle

One of the issues I see is that in a fishless cycle, we test for nitrite(NO2) as feedback that the first "A-Bac" (ammonia oxidizing bacteria (Nitrosomonas spp.)) population is growing. We test for nitrate(NO3) as feedback that the second "N-Bac" (nitrite oxidizing bacteria (Nitrospira spp.)) is growing. What I think KK is getting at and what has happened to me a fair amount too in helping beginners is that some of these bottled bacteria products seem to just directly put NO2 and NO3 in the water, thus leading to false positive test results for a fairly long period of the fishless cycle.

The dream of packaging and reviving living bacteria for use at a targeted later point in time is based on correct knowledge by bacteriologists (as far as I know, based on at least one paper) that our particular two species are among those that can form spores and later come out of the spore state. The problem seems to be that so far, just about all we ever see is the bottle contents behaving as if dead organic material and intented nourishment substances are coming out but never evidence that the bacteria are reviving.

I think that getting the bacteria to revive is a very hard problem in itself and I'm not aware of Hovanec or anyone else ever publishing on this topic. Instead, all anecdotal evidence seems to show organics going up, bacterial blooms (heterotrophs) sometimes following and very delayed spikes in ammonia eventually happening as if fairly large bunches of flake food or simply organic mulch had been dumped in. The problem then with the cycling process is that the carefully tended dosing regimen is upset, leading to typical over and underdosing problems (and again, I have to repeat that discussing this is necessarily general because I do feel the different products are pretty different from each other, not just always having bad effects but sometimes just no effect.)

I guess I'm agreeing with KK in that even though sometimes the fishless cycle appears to proceed just as if you were doing a normal ammonia fishless cycle (with the usual 1 to 2.5 month time period) there are other times when the bottled bacteria substances seem to create many weeks of confusion finally culminating is what feels like a fresh start of some sort and a subsequent normal ammonia fishless cycle (or, more often, the beginner gives up at some point during this, switches to a fish-in cycle and either loses some fish or changes a lot of water and sees no symptoms.)

Similar to KK, I've pretty much watched hundreds of these fishless cycling threads in the last few years and it's frustrating to watch one of these that gets in to a sort of double delay. I guess to add to the non-scientific mess of it all we are not good at all about cataloging which BBs (bottled bacterias) do what - we keep no tally, adding to our own blame in not being able to describe it more clearly. (Perhaps BTT will comment that he and MW and I remember a time about 3 years ago when there were a different set of BBs about that we saw some different observations of, but that's a different story from now I think.)

WD
 
Indeed I will, WD.

A couple of years ago, we seemed to be hearing more and more of a bottled bacteria product which actually worked, Marineland BioSpira. It was developed by Dr Hovanec as a result of his findings in writing his scientific papers which we often quote from here. He found that the bacteria previously thought to be the dominant N-Bacs in our aquariums (Nitrobacter spp.) Actually wasn't the dominant N-Bacs species at all. Instead, a different species turned out to be dominant species (Nitrospira spp.).

Marineland BioSpira was therefore the first and only bottled bacteria product to actually contain both correct bacteria species, and certainly seemed to work.

Dr Hovanec has patented the use of Nitrospira spp. In a bottled bacteria product in his own name and so when his partnership with Marineland ended, I assume that Marineland could no longer use Nitrospira in their BioSpira product. We heard several reports that BioSpira was no longer acheiving what it used to, and I think the product has actually now been withdrawn from sale.

There was also another product which seemed to work called Bactinnettes, however this did not contain any N-Bacs, so only helped for the first stage of the cycle, the oxidisation of the ammonia. I'm not sure if this product is still on sale. I suppose a quick google search would reveal the answer.

Interestingly enough, after Dr Hovanec left Marineland, he produced his own bottled bacteria product. From memory it was called Dr Tim's The One and Only, but I'd have to check that. I've never heard of anyone who used it so can make no comment on its effectiveness, but would very much like to. Again, a google search would likely reveal whether this product is still availale and where it can be bought from.

WD, have you read Dr Hovanec's paper where he tested Nutrafin Cycle as I mentioned above?

BTT
 
Ok guys, in the interest of proving it to myself, I've done some digging and found the research by Dr Hovanec which I spoke of above. It's actually contained within the patent document. Here is the link:-

http://www.timhovanec.com/Publications/page7/assets/hovanec_patent_US6268154.pdf

Scroll down to page 21 of the document and read the section headed up 'Commercial Additive' (starting about half way down the page in the right hand column).

Dr Hovanec clearly concludes that whilst Cycle doesn't encourage the growth of the bacteria which it actually contains, it does have a "fertiliser effect" on Nitrospira spp. bacteria, and encourages them to grow more quickly. Very interesting indeed on a forum where the general consensus is that this stuff is useless.
 
Ok guys, in the interest of proving it to myself, I've done some digging and found the research by Dr Hovanec which I spoke of above. It's actually contained within the patent document. Here is the link:-

http://www.timhovanec.com/Publications/page7/assets/hovanec_patent_US6268154.pdf

Scroll down to page 21 of the document and read the section headed up 'Commercial Additive' (starting about half way down the page in the right hand column).

Dr Hovanec clearly concludes that whilst Cycle doesn't encourage the growth of the bacteria which it actually contains, it does have a "fertiliser effect" on Nitrospira spp. bacteria, and encourages them to grow more quickly. Very interesting indeed on a forum where the general consensus is that this stuff is useless.
That was a very enjoyable read over a cup of coffee or two.

Keith.
 
As Keith said, that's a good read (only read the 'Commercial additives' :rolleyes: ) BTT.

I'm quoting this from Dr. Tim's site (hopefully not a problem?), where he explained about fishless cycling and listed 'Dr. Tim's Aquatic One and Only Live Nitrifying Bacteria' product as an option:

Using DrTim’s Aquatics One and Only Live Bacteria: The best and easiest way to fishless cycle is to combine adding the ammonium chloride with our Live Nitrifying bacteria. When used in combination these will cycle the tank in less than one week. Again, do not add too much ammonia. We make it easy by providing a bottle of reagent grade ammonium chloride that is at a concentration such that adding 1 drop of solution to 1 gallon of aquarium water will result in an ammonia-nitrogen concentration of 2 mg/L (ppm).
The procedure is to add the ammonium chloride solution, shake the bottle of nitrifying bacteria well and add it to the aquarium. Measure ammonia and nitrite the next day and record. Add ½ dose and wait 24 hours and measure again. By day 5 to 7, you should be able to add 1 drop per gallon and the next day ammonia and nitrite will be 0.

The cost of the product seems to be around the same price as other commercial products. So, not a bad option to try it. If only i knew about this when i was experimenting with Tetra SafeStart + bottled ammonia (source) :good:
:)ninja: although i definitely think safestart helped a great deal with my cycle :ninja: )
 
Interesting!

Would be great to setup and test several identical tanks at the same time to see what works best ;)
 
Interesting!

Would be great to setup and test several identical tanks at the same time to see what works best ;)

Yes it certainly would. I've always wanted to do that, but I've never had the time or the space.

Glad you guys found it interesting.

BTT
 
Yipes! Can't believe I lost track of this thread. That patent writeup is a really good find. I had never read that before, only the scienfific papers that were behind it (as you had too BTT) and I think the nice thing about this one is that Dr. Hovanec's audience are the patent examiners rather than his scientific peers, so things get explained in a way that is more understandable to us non-bacteriologists, don't you think?

BTT, I think one missing piece of the puzzle for you may be that we had a member a while back who did indeed obtain and try out the one and only product from California. I don't know whether we'll be able to dig it back up with searches but what I recall was that either the particular case of this member was just non-definitive because of the individual circumstances or that somehow in this case the BB was still just pretty ineffective for some reason.

In real terms it's probably just yet another of our usual situations that we don't have enough practical experience to allow any of us to make much of a call on it. My own worry is that while the finding of the correct strain of N-Bac is an important step, the perservation and transport issues are greater than proponents such as Tim ever want to talk about (having a business interest) and/or the actual processes that produce well-fishless-cycled aquaria still suffer from some lack of communication from practical practitioners such as ourselves versus scientists (like Tim) or industry/LFS background types (people who we know are only too ready to yield to the impatience of the customer dominating the whole issue.) (eg. many "use instructions" for BB products still seem to reveal a mix of goals that seems to be somewhere -between- fish-in cycling goals and true fishless cycling goals in my opinion.)

WD
 

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