As subadult fish, how do i tell if they are male or female? do the egg spot apear on you males?
Sexing really depends on the species, p.acei for instance can only be reliably sexed by venting or observing spawning, or some are simple to tell such as p.crabro where the females and juveniles are yellow with brown vertical stripes and males turn dark blue with darker stripes.
Egg spots aren't a reliable method of sexing, females can have them as well as males, and males lower in the pecking order may not have any at all.