First, please can you make your wife be friends with my wife.
Second, don't bother taking the water, there are very few bacteria in the water column.
Third, the bacteria live in various areas, not least the filter, but also the substrate and and decor. Because of this, there's no way to be sure that you can instantly cycle your new filter, but even if it doesn't, you'll have a huge head start in the cycling process.
The accepted wisdom is that you can take up to a third of the mature filter media, and put it in the new filter. You need to top the media in your old filter back up with new media, in order to provide somewhere for the existing filter bacteria to recolonise onto.
I would suggest doing a week of fishless cycling at 1ppm ammonia, just to check that you've got a reasonable bacteria colony in the new filter, and monitor your ammonia and nitrite levels daily when you put the guppies in.
The best ratio is 2 or 3 females per male.