The colony of bacteria is not going to die off because the fish are continually adding waste as a food source along with uneaten food. I mean no disrespect but I believe you are misunderstanding the process of the nitrogen cycle and the affect it has on the beneficial bacteria produced. Plants help a great deal in eating up ammonia but donāt get it all. The bacteria can just as quickly eat it up. They balance each other. I have a goldfish tank that always has 40 ppm nitrates by tank cleaning day ( which is once a week). It is heavily planted to help with nitrates but the plants canāt keep up with the bio load. I agree that there are differing opinions but if you stop to consider that bacteria are living creatures, then you know they have to have a food source to thrive and live. We will simply have to agree to disagree on this one. I am open to new information though.I am not the best person to be leading this discussion because my understanding is based upon my research and not as a biologist or microbiologist. TwoTankAmin is a member here who has delved into this much more deeply and could provide us with all sorts of references; he wrote the articles pinned at the top of the cycling forum, and these do recommend adding ammonia periodically. However...
I cited a paper in post #49 which determined that the AOB do not die off rapidly in the absence of ammonia; conditions factor in, as I said. But the evidence is very clear that depending upon these conditions, the ammonia can go into a sort of suspended state for weeks and even months. There is also the evidence in other studies that high ammonia levels can inhibit the NOB (nitrite oxidizing bacteria) which may explain why some people have issues with nitrite reduction during cycling; I would suspect the NOB are being inhibited if not killed by the ammonia. Even the AOB themselves can be killed by excessive ammonia.
But that is not really the point, when plants are present. Aquatic plants use ammonia/ammonium as their preferred source of nitrogen, over nitrite and nitrate. I have never cycled a fish take in my life, but I always have floating plants and lower plants in from day one, and then add the fish. If the plants were not taking up the ammonia, all my fish would never have lived past a day or two. In her book Ecology of the Planted Aquarium, Diana Walstad has dozens of citations from scientific studies on the topic.
Plants rapidly detoxify ammonia. As NH3 (toxic ammonia) enters the cell by simple diffusion across the membrane, it may combine with a hydrogen ion (H+) and convert to non-toxic ammonium (NH4+). This NH4+ can be stored in cell vacuoles. The plants use this ammonium as their source of nitrogen. One study found the vacuoles of Nitella clavata to contain over 2,400 mg/l of NH4+. Another method plants use to detoxify ammonia is to immediately use the ammonia to synthesize proteins. Toxic NH3 is combined with stored carbohydrates to form ordinary amino acids. Thus, plants that grow well can tolerate more ammonia because they have more carbohydrates to combine with ammonia [Walstad, p. 21].
I asked Tom Barr on another forum about the level of ammonia plants might be able to handle, in connection with new fish being introduced to an established tank and the aquarist was fretting over a mini-cycle. Tom said it would be almost impossible to add so many fish to an aquarium with healthy plants that the plants would not be able to rapidly handle the ammonia.
As a last thought, if you establish the AOB and NOB by fishless cycling with ammonia, and you have live plants that once they start growing will utilize most of the ammonia being produced by the fish and decomposition, would the AOB and NOB not die off for lack of ammonia/nitrite anyway? You see, it just doesn't make sense why you would waste time cycling the tank when the colony of bacteria is going to die off as soon as you have fish and plants.
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