"Hi Dr. Hovanec.
Introduction deleted.
One of our main issues:
1. How to get biofilter working at low pH?
2. Can nitrifying bacteria adapt somehow to low pH (i.e. < pH 5.0)?
3. Can a very slow adaption (several weeks/months), serve to help nitrifying bacteria cycled in an alkaline or neuter culture media, work in moderately acid media?
Hello Mr. R:
Thanks for the email - years ago (and I mean around 1986 or so) I spent several weeks exploring the entire Venezuelan Coast from Columbia all the way over the Orinoco Delta as a consultant for a company that wanted to build a shrimp farm somewhere - was a great trip. Before cell phones and the internet!
Please see my answers to your questions below and if you have any follow I would be glad to answer those also.
This basically answers the above 3 questions:
The best way would be to start at a higher pH and get the bacteria going then slowly reduce the pH. The bacteria will slow down and so you have to have lots ot patience and monitor ammonia and nitrite and keep the value below 2 ppm and let the bacteria adapt or select themselves for being able to work in the low pH environment. I am not sure how long it would take - probably months rather than weeks.
Also does your group tend to keep the fish in high humic conditions? Nitrifiers do not like humic acid or humic conditions so this is a potential problem. And if would be better to have a substrate for the bacteria to grow on like ceramic pieces etc rather than free swimming in water.
I have taken the liberty of attaching a few papers that show nitrifiers can be "adapted" to low pH values - 3.8 in one paper and it is interesting that they found large amount of Nitrospira as the nitrite-oxidizer which, of course, is what I showed years ago. So you could start with some of my One & Only as a seed and then slowly cultivate a low pH bacteria group (not mono or pure cultures). Once you had a decent population you could share amongst your group.
I hope this helps."