I have a 25l tank which has had 2 zebra danios in it for a fortnight. I have been taking readings regularly and changing the water if it reaches 0.25ppm ammonia.The tanks is currently 25 degrees c with 0.25ppm ammonia and no nitrite.Why haven't I got any nitrite readings yet if it has been running for 2 weeks? I have put in stress Zune according to the instructions.I have also left the filter alone as well as the gravel only changing the water so that the bacteria can grow but why isn't there any sign of them yet?