I started using Purigen about a month or so ago. You don't mention the size of your tank, but one 100ml pouch will treat 100g. HOWEVER, I'm using an AquaClear 70 HOB filter* (60g tank) and one pouch left significant room around it for water to bypass, so I have placed two pouches side by side so all water must pass through one or the other.
Purigen is usually good for about 6 months although the time interval is relative to the amount of disolved organics in the water that it has adsorbed so time intervals may vary significantly.
As another poster points out it is a 2 day process to regenerate Purigen - 1 day to soak in 50/50 bleach/water and another day to soak in water/conditioner to de-chlorinate. However, I don't see an issue running for a couple of days without it.
If you do purchase backups to cycle, according to Seachem Tech Support, regenerated Purigen must be kept wet in a plastic bag as once used, if it dries out it may crack and become less effective.
Purigen is awesome btw. My water is very crystal clear and reminds me of the polish I saw back in the day when I periodically ran a Vortex diatom filter.
*Note: I actually have two AC 70 filters on my tank. One as mentioned above is sponge, purigen and floss (mechanical). The other is loaded with Seachem Matrix and uses a Fluval Edge sponge pre-filter on the intake tube (biological).
regards,
AD