You can get test kits that test the O2 in the water and as long as you over 7mg/l your perfect i believe (For most fish, very few need it higher)
The filter is best on 24 hours a day or the bacteria can die of low O2 levels as the lack or flow through the media.
As long as you stocking to stocking guide lines then their should be no reason to be worried, it's only a problem when over stocking.
Sings of low O2 are all fish gasping at the surface, bottom dweller's darting up gulping and dashing down again. Fish hanging around aeration bubbels and flows of water, as these are the best places to first get the disolved O2.
Helter