A good question, and one I can't answer. As you realise, fish tend to measure territories by surface area of substrate not volume. In this case, I would suspect a couple of puffers would be fine if introduced together and as juveniles. It can help to divide the tank up with visual markers, in otherwords, have two piles of rocks, one at each end, and an open space of sand in between. Hopefully, the fish will use that sandy space as the boundary between their territories.
Realistically though, almost no aquarium is as big as the territory fish hold in the wild. Even something as small as dwarf cichlid will hold the equivalent of a space 60 cm by 60 cm (about 4 square feet). So most aquaria end up being 'cramped' by the standards of what fish expect in the wild. Possibly exceptions might be some gobies and killifish, but puffers, like cichlids, probably don't find home aquaria nearly big enough.
Cheers,
Neale