As long as the replacement water closely matches the tank water you should not have a problem. What you should really do is concentrate your efforts on what is causing the ammonia reading.
***Edit To Add***
Your tank water contains a tiny percentage of nitrifying bacteria. I do daily 80%+ water changes on fry tanks the first week or two, and set up tanks with cycled filters & 100% fresh water when growing out spawns.
Your nitrifying bacteria is in your filter media, not the water.
hi damovts if you do an 80% water change and syphon every bit of dirt out you are more likely to put the nitrite and amonia higher then what it is ,like someone said above you'll be doing a mini cycle.the best thing is do a 40% water change one day then keep doing 25% water changes after that everyday if you can it will go down just have a little patience also if you are given to much food to your fish then cut down a little better to give small potions 3 or 4 times a day then given big potions.hope this helps i know its annoying it took my tanks amonia to go down a while but eventually it did go down just took a bit time and patience![]()
Getting it out of the gravel that isI sure would like to see some documentation showing that doing large water changes results in cycling problems, or that removing debris from the gravel causes cycling problems. Most of my tanks have no substrate, when debris is removed there is nothing but clean glass.
If this cause ammonia & nitrite spikes I would have hundreds of dead or deformed angel fry, dead breeders, and so on. The few tanks that do have substrate get 50% water changes, with a vac, and experience no problems.
Large water changes, especially with cooler water, are one method used to induce fish to spawn. It is generally accepted that spawning fish are happy with their environment, or they would not be healthy enough to reproduce, nor sense that the environment is right for reproducing.

I sure would like to see some documentation showing that doing large water changes results in cycling problems, or that removing debris from the gravel causes cycling problems. Most of my tanks have no substrate, when debris is removed there is nothing but clean glass.
If this cause ammonia & nitrite spikes I would have hundreds of dead or deformed angel fry, dead breeders, and so on. The few tanks that do have substrate get 50% water changes, with a vac, and experience no problems.
Large water changes, especially with cooler water, are one method used to induce fish to spawn. It is generally accepted that spawning fish are happy with their environment, or they would not be healthy enough to reproduce, nor sense that the environment is right for reproducing.
No not really, you would get rid of too much of the beneficial bacteria and cause a mini cycle.