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fluttermoth said:Loads of people do it
Just be very careful as some batting (and cushion/pillow/toy stuffing/fillers) is treated with a flame retardant, which is toxic to fish. Personally, I buy a big roll of proper filter floss (well, I bought one roll; I've still got most of it left!), as I don't have to worry then.
fluttermoth said:Yes, your good bacteria will survive, if you keep the media wet.
Sponge is a very good home for bacteria (floss isn't) and it doesn't need replacing (floss does!). Floss is really only there as a mechanical media, to physically remove any fine particles from the water. You only need a thin layer of floss, somewhere in the filter, if you use it at all; not everyone does.