i think i often use more than is instructed on the bottle. the reason being that you are meant to use one small capful for 45 litres. these cap measures really are small so when i'm doing my 25% water change, i'm replacing about 25 - 30 litres. therefore i have to measure half a small capful. and to make matters worse, the bucket i am using (until i can find a more suitable larger one) can't hold all at once so i have split this into 3 10 litre additions. so now i'm down to a quarter of a small capful each time! this is a minute amount and it's really hard to measure as theres no measurement guides on the cap. never mind it's all good fun!
in answer to the question, i've yet to see any harmful effects on my fish