The fact that little of what I said made any sense to you is because you have no real idea what your are doing. My attempt to explain this has clearly failed.
What makes the situation even worse is that you apparently did not spend any time at all trying to figure out any of it. So before I walk away from this thread I will do a bit of the work you should have done yourself. The information below is from the University of Florida:
Most commercial ammonia test kits measure the total ammonia nitrogen (TAN). Again, it is the un-ionized ammonia (or UIA) portion of the TAN that is more toxic. The UIA fraction of the total TAN can be determined from the TAN measurement if you know the temperature and pH of the water. At high temperatures and high pH, there is more UIA. Therefore, a good ammonia test kit will include a TAN test, a pH test, and a thermometer........
Interpreting the Ammonia Test In healthy ponds and tanks, ammonia levels should always be zero. Presence of ammonia is an indication that the system is out of balance. Therefore, any ammonia in a pond or tank should alert the producer to start corrective measures. Un-ionized ammonia (UIA) is about 100 times more toxic to fish than ionized ammonia. This UIA toxicity begins as low as 0.05 mg/L, so the result of the TAN test needs to be further calculated to find the actual concentration of UIA. To do this calculation, the temperature and pH need to be measured.
from
http/edis.ifas.ufl.edu/fa031
The API kit does not measure total ammonia nitrogen (TAN), it measures total ammonia (TA). The measurement scales are not the same. Its like miles vs kilometers. 5 ppm of total ammonia nitrogen will read 6.4 ppm total ammonia on an API kit. This is amplified as one tests next for nitrite and finally for nitrate.
or this:
REVIEW PAPER
Ammonia in estuaries and effects on fish
F. B. EDDY
Environmental and Applied Biology, Faculty of Life Sciences, University of Dundee,
Dundee DD1 4HN, U.K.
(Received 3 December 2004, Accepted 18 July 2005)
In many studies the toxicity of ammonia is described in terms of ambient unionized ammonia since this is the variable that correlates with toxicity (Alabaster & Lloyd, 1982; Thurston et al., 1984). This criterion, however, is based almost exclusively on
data from freshwater fish that are considered to be relatively impermeable to NH4+. Since seawater fish apparently have enhanced permeability to NH4+ it may be inappropriate to refer to toxicity in terms of unionized ammonia alone (Wilson & Taylor, 1992; Wilkie, 1997). It may be for this reason that ammonia appears to be more toxic in sea water compared to fresh water. This is supported a recent analysis by Ip et al. (2001) and Randall & Tsui (2002) of the data presented by USEPA (1984, 1989) that indicated that the mean acute toxicity value for 32 freshwater species was c. 2.3 mg NH3–N 1-1compared with 1.5 mg NH3–N 1-1 for 17 seawater species. For the five most sensitive species the values were 0.79 mg NH3–N 1-1 and 0.68 mg NH3–N 1-1 for fresh water and sea water respectively. Generally salmonids are amongst the most sensitive species and carp and cyprinids are amongst the least sensitive to ammonia (Seager et al., 1988; USEPA, 1999). As previously mentioned, however, differences in ammonia sensitivity between species are much greater than differences related to salinity.