Its only 24hours as far as I know cos that's roughly how long it takes to warm up cold water to the right temperature. Dont leave it any longer than this otherwise ur bacteria will die. Also allows time for any ammonia left to be converted as well. U dont want ammonia / nitrite present when you add ur fish. If you add water you've already warmed up by having a spare heater in the bucket etc. then I'm sure you could add fish almost straight away taking in consideration what I mentioned above.does it have to be 24 hours or could i do it in the morning wait for the water to get up to temp then get the fish in the afternoon?