Are you sure that the nitrite is ZERO? With a used filter, assuming the media was kept wet the whole time, and it wasn't too long between when it was last used and when you started dosing ammonia, you could have a pretty nice colony of bacteria there already. Did the drops turn purple in the bottom of the tube before you shook it and then it turned a weird grayish color? If so that is a reading "off the scale" meaning far above 5 ppm.
It didn't turn purple at all, just straight to the pale blue. I am going to re-test in a moment, and test for nitrates as that must be where it all is at!
The filter was being used in a fish-in cycle, that I wasn't happy with so that's why it's here so I guess it's possible that it had some helpful bacteria (it was transferred straight to this tank from my other tank with fish-in).
Anyway, now the emergency is out of the way (my mrs just found a snail in the fishless cycling tank and has forced me to put it in the other tank