MMMM as for most of the bacteria living in the sponges of the filter im confused now.I have 2 Dolfyn power filters running which I removed the carbon filters from and just added ruf cotton.( Almost like a sponge )In the filter there is what they call a biofilter which apparently host bacteria but its just normal plastic so i dont really see how this works.Now I replace those sponges regulary with new ones and through the others away.Is this not correct then and do most bacteria not live in the substract? Confused
If you have an under gravel filter, then a lot of your bacteria will live in the gravel.
If you have an internal or external filter, the vast majority of the bacteria grows on the filter media, be it sponges or ceramic madia or noodles etc.
Hardly any beneficial bacteria will live in a sand substrate, and you wouldn't want it to because your water is not passing through it regularly to get treated.
I dont know what type of filter you have d3tained, but generally its not wise to regularly change your sponges - this is where your bacterial colony will likely exist and many sponges can last for years. They just need a gentle rinse in old tank water to remove the worst of the gunk then pop them back in.