amonia is 0Water parameters please?
Ok so plan is daily water changes til get a zero nitrite reading.If nitrite reads more than zero a water change is needed to get it down. With a nitrite spike the best way to treat it is test every day and do a water change when it reads above zero.
Was there a heater in the breeding box? And after you returned it to the main tank did you finish with a water change? It’s possible there was a temperature shock if the breeding box didn’t have a heater or had it set to a different temp and was simply dumped in the main tankhave had the oto in my breeding box for a couple of days and after the water change it seemed better, BUT we did loose a endler guppy yesterday.
I am going to do another 20% water change after school run. Out of all my oto's this one seems to be the smallest.