I would set the tank up and get it started running with the filter for it. Once the water has settled and cleared, I would bag my fish (you will probably have to take a lot of the water out of the 20 gallon to catch them all and 2 nets work great for catching fish), move the filter over from the 20 gallon to the 55 gallon, start it running and then start acclaimating the fish. The filter won't be running for more than an hour without fish in the tank and that won't be a problem at all.