The ammonia oxidizing bacteria have no problem in the high 80sF (over 30C). The nitrite ones are optimal a bit cooler, but should not be in bad shape higher either. So my fist question is still, if the ammonia readings were real, where are the nitrite readings?
So if we can assume that the rise in temp did not inhibit the bacteria, would there not need to be another explanation from where the readings came?
If one wants to suggest that in warmer water oxygen levels can be lower and this may have inhibited the bacteria, two questions immediately arise. What about the fish, shouldn't low oxygen also affect them? Then, back to nitrite, low oxygen would affect nitrite as well as ammonia bacs. So, again, where is the nitrite? And then why shouldn't one's normal setup keep the water oxygenated at a temp a few degrees higher?
I have been trying to find research on the effect of temperature on ammonia tests with no luck so far.
Here is an anecdotal piece of evidence. A while back I had a heater stick on. It raised the tank temp to about 105F. It killed the discus and rummy nose, but all the L450 plecos survived. Now I can understand this from a purely temp point of view because these fish can handle pretty warm water. Ammonia and nitrite is another story. So here was a tank where the temps got much hotter than those in this thread. Hot enough to kill fish used to warmish waters. Why didn't ammonia rise to the point of harming the plecos? these fish spawned many times since then too.