The tank with its current occupants has been running about eight months. The two Koi was pulled in from my pond for the winter (Northeastern US) because last year (winter 02/03) only four of seven survived the pond freezing solid and the day before the move I saw a heron standing in the pond and then there were two. His cohort went a few days after the indoor move and I suspect gravel or transition shock got him. Thinking the remaining one might get lonely I bought the plecos (which were then tiny). In the spring I got lazy and kept all inside. The water is from the same well I use for drinking water and is the same stuff used to fill the artificial pond all was started in a few years ago. A little lime in it, but that's all. (Yes, it has been tested periodically). The PH is low. Ammonia is monitored with bi weekly litmus testing and a bio alert stickon. The usual white cloudiness every three or five days causes frequent water changes. Under a microscope the cloudiness seems to be a micro organism (mostly ameoba) bloom. Changes of water control that. There is a Cascade brand filter pump, but months ago I gave up on the expensive and quickly fouled sponges that we are supposed to buy and use glass wool (which is much cheaper) instead as the primary medium and a bag of 50/50 mix of rosin & charcol to give good bacteria a place to live. The filter inlet was extended back in January with a hose to the bottom. Observation showed that was where the crud collected and a bottom inlet sucks up what an upper inlet can't. The pump box itself is cleaned every two weeks. The glass wool is replaced whenever it gets dirty looking. There were no observed problems before suddenly finding one of the Plecos dead and bloody. Needless to say, I am not a happy camper and want to know what happened.