How long can BB survive?

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Hi, all. I'm rebuilding my 150g, and shut off the filter today. This is a long-established tank, and it was fairly densely stocked. I'd like to order new fish soon and not cycle again. Does anyone know (science only, please, no wild guesses! :) ) (@Byron , this is your sort of thing) how long I have before I'll have to cycle?

Are there ways to keep the BB alive for a couple days without running the filter? It will be at least this time tomorrow before I can turn on the filter again.
 
Your BB will be fine for at least 48 hours. I have moved tanks all over the country and have never had a problem. Temperature is a factor, don't have it to warm the more heat the more work and oxygen they consume. I have always put my base medium into 15 liter buckets and placed them outside when re building tanks.
 
Depending on the filter and if the filter is kept running, the bacteria might last an hour or several days.

If you have an external canister filter and it is turned off, the bacteria will start to die after an hour or two. If the filter is clean (was cleaned in the last few days), the bacteria will last longer but will not last more than 24 hours without water flowing through the filter.

If you have a HOB filter like an AquaClear hang on the back, and the filter has been cleaned within the last few days, the bacteria can last a week or so because the top of the filter is open and air can get in to keep the oxygen level high enough for some of the bacteria. Some will die but some will survive.

If the filter is dirty and full of gunk, the good bacteria will die within a few hours because the gunk will hold lots of bad bacteria that competes for oxygen.

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If you have a power filter, you can clean the filter media and put the media in a bucket of tank water. Have an air pump bubbling air into the bucket and the bacteria will last for weeks. However, it will go dormant after about 2 weeks without food (ammonia).

You can also have a bucket of tank water and put the power filter on that and let it run. Then the bacteria will last for several weeks before it goes dormant.
 
I thought you were talking about the substrate not the filter itself. The filter BB will start dying off almost immediately once water stops flowing threw it.
 
Well. I'll try to do something tomorrow morning first thing with it. I'll put the filter media in a 10 gallon tank I have and get an air stone going; maybe I can save enough of it. Once the tank is up and running again this weekend, I'll add some ammonia, do a test, and see if the Beneficial Bacteria (@I Like Rare Fish ) is still alive and kicking.
 
You asked me, so I will respond by saying I agree with Colin (post #3). It is not, as many think, the lack of "food" (ammonia) that kills the nitrifiers; they can live without "food" longer than many realize, by going into what Colin termed a dormant state, or some call hibernation. Whatever the name, the point is the bacteria do not die, unless from the other issues associated with this. I wrote an explanation some years ago that may provide some background, though I will mention that I believe some newer data may be challenging some of this now, but it would take me some time to track this down and study it, so I'll leave it as written. There is another issue, and that is that after the initial period, it is more probably archaea and not bacteria that deal with nitrification. That too is another complex issue which I have not had time to delve into as much as I should, so I will just mention it. Always having live floating plants in my tanks, I have never fussed over bacteria/archaea.

Nitrifying Bacteria
Nitrification is the oxidation of ammonia/ammonium to nitrite and then the subsequent oxidation of nitrite to nitrate; this is performed by two groups of bacteria known collectively as nitrifying bacteria or nitrifiers. True nitrifying bacteria are autotrophs; they use chemosynthesis to manufacture their energy by using oxygen plus nitrogenous waste (ammonia or nitrite) and carbon (from CO2). There are several different bacterium species involved, all in the family Nitrobacteraceae, that carry out this function in soil, and it used to be thought that these, particularly Nitrosomonas europa and Nitrobacter, were the nitrification bacteria in freshwater. But Dr. Timothy Hovanec led the team of scientists that proved this to be a mistaken assumption. Ammonia is converted to nitrite by bacteria of the Nitrosonomas marina-like strain [2] and nitrite is converted to nitrate by bacteria closely related to Nitrospira moscoviensis and Nitrospira marina. [3] With several subsequent scientific studies by other scientists on wastewater nitrifying bacteria this data is now accepted.​
Once established, the population of these bacteria in an aquarium will be in direct proportion to the amount of ammonia or nitrite respectively. Nitrifying bacteria require 12-32 hours to multiply, which they do by binary division [each bacterium divides into two bacteria]. Nitrosomonas multiply in less time (12+ hours) while Nitrospira require more time (up to 32 hours). In a new aquarium, it can take up to eight weeks for the bacteria populations to reach a level capable of eliminating ammonia and nitrite.​
Scientific studies have also now proven that Nitrospira are inhibited and cannot multiply in water that contains significant concentrations of ammonia, and evidence exists to suggest that existing populations of Nitrospira actually become dormant when ammonia is present in high concentrations. Kim et al. (2006) determined that with an active ammonia [NH3] level of 0.7 mg/l (=ppm) Nitrospira bacteria experienced a decrease of 50% effectiveness, resulting in an accumulation of nitrite. [4]​
The pH has a direct effect on nitrifying bacteria. These bacteria operate at close to 100% effectiveness at a pH of 8.3, and this level of efficiency decreases as the pH lowers. At pH 7.0 efficiency is only 50%, at 6.5 only 30%, and at 6.0 only 10%. Below 6.0 the bacteria enter a state of dormancy and cease functioning. [5] Fortunately, in acidic water (pH below 7.0) ammonia automatically ionizes into ammonium which is basically harmless. And since nitrite will not be produced when the ammonia-oxidizing bacteria are in “hibernation,” this decrease in their effectiveness poses no immediate danger to the fish and other life forms.​
Temperature also affects the rate of growth of nitrifying bacteria. It will be optimal at a temperature between 25 and 30C/77 and 86F. At a temperature of 18C/64F it will be 50%. Above 35C/95F the bacteria have extreme difficulty. At both 0C/32F (freezing) and 100C/212F (boiling) the bacteria die.​
These bacteria cannot survive drying out; without water, they die. Tap water with chlorine may kill these bacteria, depending upon the level of chlorine and the duration of time the bacteria are exposed to it. Some antibacterial medications may negatively impact the nitrifying bacteria to varying degrees.​

[Edit today--as mentioned above, there may be contrary evidence on the species, plus the archaea rather than bacteria issue.]​
Endnotes:
[2] Paul C. Burrell, Carol M. Phalen, and Timothy A. Hovanec, “Identification of Bacteria Responsible for Ammonia Oxidation in Freshwater Aquaria,” Applied and Environmental Microbiology, December 2001, pp. 5791-5800.​
[3] Hovanec, T. A., L. T. Taylor, A. Blakis and E. F. DeLong, “Nitrospira- Like Bacteria Associated with Nitrite Oxidation in Freshwater Aquaria,” Applied and Environmental Microbiology, Vol. 64, No. 1, pp. 258-264.​
[4] Kim, D.J., D.I. Lee and J. Keller (2006), “Effect of temperature and free ammonia on nitrification and nitrite accumulation in landfill leachate and analysis of its nitrifying bacterial community by FISH,” Bioresource Technology 97(3), pp. 459-468.​
[5] Kmuda, “Aquarium Bacteria and Filtration Manifesto,” Parts 1 and 2, OscarFish website.​
 

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