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Dr. Tim'S One & Only Nitrifying Bacteria Does It Work?


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#1 rebrn

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Posted 10 October 2009 - 12:39 AM

Hello, I have discovered a new product (well new to me) called One And Only Nitrifying Bacteria H2O-PURE by Dr. Tim's Aquatics. All the reviews say it works great, it was developed by the same person who made Bio-Spira. Has anyone actually used the One and Only product and does it work. It is not all that expensive but I still don't want to throw away money.

Thanks

****UPDATE****

Ok for everyone who is interested if this product works ... It just arrived. I will be using it in two tanks a 12 US gallon and a 5 US gallon:

Tank Information:

12 Gallon - never had fish ... brand new tank, filter and gravel.

PreDose: Ammonia - 4 ppm, Nitrites - 0 ppm, Nitrates - 0 ppm, pH - 7.8, temp - 86 F (roughly 30 C)

5 Gallon - fish-in to fishless convert cycle (fish in for 1.5 months then died - jumped out of tank) ... otherwise brandnew tank, filter and gravel. It is possible that there may be some bacteria already in this tank but unlikely, had 2 very minor nitrite spikes during fishless cycle (0.25 ppm) gone the next day think the presence of nitrite was user error with test. <img src="http://www.fishforum...>/rolleyes.gif" style="vertical-align:middle" emoid=":rolleyes:" border="0" alt="rolleyes.gif" />

Pre-Dose: Ammonia - 4 ppm, Nitrites - 0 ppm, Nitrates - 0 ppm, pH - 7.4, Temp 86 F (roughly 30 C)

Although it states you cannot overdose this product both tanks were dosed with the minimum reccommended dose (1ml/US gallon) at 3:30 pm EST.

I will continue to update, in first post, with the every 12 hour test results.


At 12 hours post dose - only change in results was a pH drop in both tanks:

12 Gallon - ammonia 4, nitrite 0, nitrate 0, pH 7.4
5 Gallon - ammonia 4, nitrite 0, nitrate 0, pH 7.2

At 24 hours post dose - no chage in any results

12 gallon ammonia 4, nitrite/nitrate 0, pH 7.4
5 gallon - ammonia 4, nitrite/nitrate 0, pH 7.2

Day 2 - 36 hours post dose (done a little early work hours change this week):

12 gallon - ammonia 2 ppm (looking good), nitrite 0ppm, nitrate 0 ppm (could be a slight nitrate reading - very dark yellow possible slight orange or maybe wishfull thinking <img src="http://www.fishforum...DIR#>/wink.gif" style="vertical-align:middle" emoid=";)" border="0" alt="wink.gif" /> ) pH 7.4
5 gallon - ammonia 4 ppm, nitrate/nitrite 0 ppm pH 7.4

Day 2 - 48 hours post dose

12 gallon - ammonia 2 ppm, nitrite 0 ppm, nitrate 0 ppm (definate this time no hint of orange), pH 7.4
5 gallon - ammonia 4 ppm, nitrite 0 pmm, nitrate 0 ppm, pH 7.4

Day 3 60 hours post dose

12 gallon - ammonia 2, nitrite/nitrate 0, pH 4
5 gallon - ammonia 2, nitrite trace - dark blue/very pale purple colour, nitrate 0, ph 7.4

Day 3 - 72 hours post dose

12 gallon - ammonia 2, nitrite possible trace (slightly darker blue then 0), nitrate 0, pH 7.4
5 gallon - ammonia 2 nitrite trace (dark blue/pale purple), nitrate 0, pH 7.4

Day 4 - 84 hours post dose (done a little early - work schedule)

12 gallon - ammonia 2, nitrate 0, nitrate 0, pH 7.4
5 gallon - ammonia 2 nitrate definate trace (not quite purple enough to call 0.25), nitrate 0, pH 7.4

Day 4 - 96 hours (sorry to those following for the late post)

12 Gallon - ammonia 2, nitrite 0, nitrate 0, pH 7.4
5 gallon - ammonia 2, nitrite trace, nirate 0, pH 7.4

Day 5 - 108 hours
12 gallon - ammonia 2, nitrite 0, nitrate 0, pH 7.4
5 gallon - ammonia 2, nitrite trace, nitrate 0, pH 7.4

Day 5 - 120 hours
12 gallon - ammonia 2, nitrite 0, nitrate 0, pH 7.8
5 gallon - ammonia 2, nitrite trace, nitrate 0, pH 7.4

Day 6 - 132 hours

both tanks - ammonia 2, nitrite 0, nitrate 0, pH 7.4

Day 6 144 Hours

Both Tanks - ammonia 2, nitrite 0, nitrate 0, pH 7.4

Day 7 156 Hours

both tanks - ammonia 2, nitrite 0, nitrate 0, pH 7.4

Day 7 168 hour

no changes in eithor tank - added additional dosage per Dr. Tim's email suggestion

Day 8 180 Hours

12 gallon - ammonia 2, nitrite 0, nitrate 0 ph 8.0 (jumped quite a bit)
5 gallon - ammonia 2, nitrite 0, nitrate trace, ph 8.0 (again large jump)

Day 8 192 Hours

12 gallon - ammonia 2, nitrite 0, nitrate possible trace ph 7.8
5 gallon - ammonia 2, nitrite 0, nitrate 5, ph 7.8

Day 9 204 hours

12 Gallon ammonia 2, nitrite 0, nitrate trace, pH 8.0
5 gallon ammonia 2, nitrite 0, nitrate 5 ph 7.8

Day 9 216 hours

12 Gallon ammonia 2, nitrite 0, nitrate definate trace, pH 8.0
5 gallon ammonia 2, nitrite 0, nitrate 5, ph 7.8

Day 10 228 h

12 Gallon ammonia 1 (dosed back to 4-ok as added extra dose of bacteria now previously), nitrite 0, nitrate trace, pH 8.0
5 gallon ammonia 2, nitrite 0, nitrate 5, ph 7.8

Day 10 240 h

12 - ammonia 3, nitrite 0, nitrate trace ph 8
5 - ammonia 2 nitrite trace nitrate 5 ph 7.8

Day 11 252 h
12 - ammonia 2, nitrite 0, nitrate trace, ph 8
5 - ammonia 2 nitrite 0.25, nitrate 5, ph 7.8

Day 11 264h
12 - ammonia 2, nitrite 0, nitrate trace, ph 8
5 - ammonia 2 nitrite 0.25, nitrate 10, ph 7.8

Day 12 276h
12 - ammonia 2, nitrite 0, nitrate trace, ph 8
5 - ammonia 2 nitrite 0.25, nitrate 10, ph 7.6

Day 12 288h
12 - ammonia 2, nitrite 0, nitrate trace, ph 8
5 - ammonia 2 nitrite 0.25, nitrate 10, ph 7.4

Day 13 300h
12 - ammonia 2, nitrite 0, nitrate trace, ph 8
5 - ammonia 2 nitrite 0.25, nitrate 10, ph 7.4

Day 13 312h
12 - ammonia 2, nitrite 0, nitrate trace, ph 8
5 - ammonia 2 nitrite 0.25, nitrate 10, ph 7.4

Day 14 324h
12 - ammonia 2, nitrite 0, nitrate trace, ph 8
5 - ammonia 2 nitrite 0.25, nitrate 10, ph 7.4

Day 14 348h
12 - ammonia 2, nitrite 0, nitrate 0-5, ph 8
5 - ammonia 1 nitrite 0.5-1, nitrate 10-20, ph 7.4

Day 15 360h
12 - ammonia 2, nitrite 0, nitrate 0-5, ph 8
5 - ammonia 1 nitrite 0.5-1, nitrate 10-20, ph 7.4

Day 15 372h
12 - ammonia 2, nitrite 0, nitrate 0-5, ph 8
5 - ammonia 1, nitrite 1, nitrate 20, ph 7.4

Day 16 384h
12 - ammonia 2, nitrite 0, nitrate 0-5, ph 8
5 - ammonia 0.5, nitrite 1, nitrate 20, ph 7.4

Day 16 396h
12 - ammonia 2, nitrite 0, nitrate 0-5, ph 8
5 - ammonia 0.25, nitrite 1, nitrate 20-40, ph 7.4

Day 17 408 h
12 - ammonia 2, nitrite 0, nitrate 0-5, ph 8
5 - ammonia 0.25, nitrite 0.5- 1, nitrate 40, ph 7.4

Day 17 420h
12 - ammonia 2, nitrite 0, nitrate 0-5, ph 8
5 - ammonia 0.25, nitrite 1, nitrate 40-80, ph 7.4 - dosed ammonia back to 4 ppm

Day 18 432 hours
12 - ammonia 2, nitrite 0, nitrate 5, ph 8
5 - ammonia 2, nitrite 5, nitrate not tested previous was 40-80 decided not to test to save reagant, ph 7.4

Day 18 444 hours
12 - ammonia 2, nitrite 0, nitrate 5, ph 8
5 - ammonia 0.5, nitrite 5, nitrate ND, ph 7.4

Day 19 AM
12- ammonia 2, nitrite 0, nitrate 5, ph 8.2
5- ammonia 0.25, nitrite 5, nitrate 80, ph 7.4

Day 19 PM
12 - ammonia 2, nitrite 0, nitrate 5, ph 8.2
5 - ammonia 0, nitrite 0.25, natrate 0, ph 7.4 (dosed with ammonia)

Day 20 AM
12 - ammonia 2, nitrite 0 nitrate 5 ph 8.2
5 - ammonia 2, nitrite 1, nitrate >80 ph 7.4

Day 20 pm
12 - ammonia 2, nitrite 0 nitrate 5 ph 8.0
5 - ammonia 1, nitrite 5, nitrate ND ph 7.4

Day 21 AM
12 - ammonia 2, nitrite 0 nitrate 5 ph 8.2
5 - ammonia0.25, nitrite 5, nitrate 160 ph 7.4

Day 21 PM
12 - ammonia 1-2, nitrite 0 nitrate 5 ph 8.2 (change from gravel to sand with no water change)
5 - ammonia 0, nitrite 0, nitrate ND ph 7.4 (dosed to 4ppm change from gravel to sand with no water change)

Day 22 AM
12 Ammonia 1-2, nitrite 0, nitrate 5, ph 8.0
5 ammonia 0, nitrites 0.5-1, nitrate 160 ph 7.4 (dosed to 4ppm ammonia)

Day 22 PM
12 - ammonia 1, nitrate 0, nitrate 10, ph 7.8 (yeah something happing)
5 - ammonia 0, nitrate 5, nitrate nd, ph 7.4 (accidental major overdose of ammonia see thread "OMG can't believe I did this" - multiple water changes ammonia 4ppm, nitrate 0, nitrate 0 ph, 8.2)

Day 24 am
12- ammonia 1, nitrite 0, nitrate 10, ph 7.8
5 ammonia 1-2, nitrite 5, nitrate 10-20, ph 7.2-7.4

day 24 pm
12 ammonia 1 nitrite 0 nitrate 10 ph 8
5 ammonia 1 nitrite 5 nitrate nd ph 7.4

Day 25 am
12 ammonia 1 nitrites 0 nitrates 10 ph 7.4
5 ammonia 0, nitrite 0.25 nitrates nd ph 7.4 (dosed ammonia 4ppm)

Day 25 pm
12 ammonia 1 nitrites 0 nitrates nd ph 7.8
5 ammonia 2 nitrites 5+ nirates nd ph 7.4

Day 26 am
12 ammonia 1, nirites 0 nitrates nd ph 8
5 ammonia 1 nitrites 5+ nirates nd ph 7.0

Day 26 pm
12 ammonia 1 nitrite 0-0.25 nitrates 10 ph 8
5 ammonia 0.5 nitrite 5 nitrate 40 ph 7

Day 27 am
12 ammonia 0.5-1 nitrite 0.25 nitrates 10-20 ph 8
5 ammonia 0 (dosed to 4) nitrite 5 nitrates 40 ph 6.8 (having to watch that my first drop below 7)

Edited by rebrn, 13 November 2009 - 02:04 PM.


#2 OldMan47

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Posted 10 October 2009 - 02:26 AM

Tim Havonec is a person that did a lot of research on nitrifying bacteria and was once involved to some degree in producing Biospira. He does have the right knowledge about the bacteria that are needed for cycling but I have no experience with his product.

#3 rebrn

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Posted 10 October 2009 - 06:38 PM

Tim Havonec is a person that did a lot of research on nitrifying bacteria and was once involved to some degree in producing Biospira. He does have the right knowledge about the bacteria that are needed for cycling but I have no experience with his product.

Ok thanks, It is only $10 for a 2 oz bottle (treats 30 US gallons) so maybe I will give it a try and see I will keep everyone posted.

#4 rebrn

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Posted 11 October 2009 - 07:19 PM

Hello everyone,

For anyone who is interested I have spoke to several people who have used Dr. Tim's One & Only Nitrifying Bacteria, the freshwater formula, and they say it works great. They have used it for establishing and re-establishing tanks, one person even used it to store his bio-media when he was making a move across country. All reports says it works and works extremely well. They also say that unlike some products you do not have to keep dosing the tank to have the bacteria colony stay, so in other words a one time dose does the trick. I have ordered some and will let you know my experience with it.

Thanks

#5 drobbyb

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Posted 12 October 2009 - 02:02 AM

Le tme know how it works. If it dosen't work, I can donate that media to you.

#6 rebrn

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Posted 12 October 2009 - 05:32 AM

Le tme know how it works. If it dosen't work, I can donate that media to you.

I will let you know, I just ordered it today so it probably won't be in untill later this week, I am guessing thursday or friday, but I will definately let you know, it would be great if it works, an actual "fast cycling" product that really works will be a welcome to a lot of people I am sure. Will keep you posted.

#7 migthegreek

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Posted 14 October 2009 - 12:45 PM

It would be great to know if there's something that can help me get started quicker.

#8 fatheadminnow

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Posted 14 October 2009 - 01:03 PM

Keep us all posted! The reason it is only one dose, that is if it is anything like Bio Spira, is because the package contains actual live bacteria. So, you just add the bacteria to your filter, one time only, so there is no need to dose daily or weekly or whatever other products claim.

-FHM

#9 rgrrmg

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Posted 14 October 2009 - 01:19 PM

Keep us all posted! The reason it is only one dose, that is if it is anything like Bio Spira, is because the package contains actual live bacteria. So, you just add the bacteria to your filter, one time only, so there is no need to dose daily or weekly or whatever other products claim.

-FHM

I would also love to know if it works, I bought this Top Fin Bacteria Supplement at my lfs and I don't think it's doing any good.

Edited by rgrrmg, 14 October 2009 - 01:19 PM.


#10 fatheadminnow

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Posted 14 October 2009 - 01:26 PM

Keep us all posted! The reason it is only one dose, that is if it is anything like Bio Spira, is because the package contains actual live bacteria. So, you just add the bacteria to your filter, one time only, so there is no need to dose daily or weekly or whatever other products claim.

-FHM

I would also love to know if it works, I bought this Top Fin Bacteria Supplement at my lfs and I don't think it's doing any good.

Yeah, it probably does not work. There really has not been anything that works, that I know of, since Bio Spira.

-FHM

#11 rebrn

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Posted 17 October 2009 - 07:55 PM

It has arrived ... let the experiment begin. I will be updating results daily in the first post.

#12 fatheadminnow

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Posted 17 October 2009 - 11:14 PM

This should be interesting!

-FHM

#13 rebrn

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Posted 18 October 2009 - 08:09 PM

after 24 hours just some minor pH changes .... full result in first post.

#14 rebrn

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Posted 20 October 2009 - 06:55 PM

well I think it might be working, ammonia definately dropped from 4 to 2 ppm in both tanks after 36 hours, trace amounts of nitrite showing in the 5 gallon at around 60 hours post dose. Still too early to say for sure, but I think something is definately happening.

#15 waterdrop

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Posted 20 October 2009 - 09:04 PM

Thanks for the updates in the first post. ...following along...

~~waterdrop~~

#16 rebrn

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Posted 22 October 2009 - 12:38 AM

Hmm ... seem to be at a stand still here no changes in 48 hours ... just when I was starting to have hope for the product .... well we will see what the next test brings

12 hour testing updates in first post.

#17 rebrn

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Posted 24 October 2009 - 02:13 AM

hello, I just posted the last few water checks ... sorry for the delay couldn't get on to the fourm I am assuming for the lay out change ... nice new look by the way.

#18 rebrn

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Posted 24 October 2009 - 04:13 AM

Well guys it has been 7 days ... what is your opinon? Do you think it is working? I am not sure if it is or not...I am thinking that if it is working it is not working fast enough for me, or am I just expecting too much? ;)

(all updates in first post)

Edited by rebrn, 24 October 2009 - 04:13 AM.


#19 fatheadminnow

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Posted 24 October 2009 - 04:26 AM

Personally I do not think it is working all that good. I mean, it may be working, but like you said, it is not working fast enough. I have seen fishless cycles, without any aids, have ammonia drop to zero within the first 8-9 days.

-FHM

#20 rebrn

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Posted 24 October 2009 - 04:43 AM

I agree FHM, mind you my 12 gallon tank sat in a fishless cycle with no aids for 2 weeks with no change at all ... ammonia started at 4ppm and stayed at 4ppm. The only activity I saw in eithor of my tanks was a 50% reduction of ammonia AFTER I added the bacteria product, so I don't know ... maybe it is just me. The last time I had a tank 10 years ago it was a distaster and I gave up ... maybe this very slow cycle is a sign ... I am not meant to have fish, but darnet I am bound and determined to break my bad luck with aquariums :lol:

#21 OldMan47

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Posted 24 October 2009 - 01:57 PM

I am sorry to find that we have yet another product that is not working, at least not the sample you got. I regularly spike new filters with the right bacteria by cleaning an old filter in a new tank. With the amount of time you have already given yours, I would be fully cycled using that method. It looks to me like the stuff you used did not have many of the right bacteria in it to get you going. It may have had some, but not enough to really give much of a leg up.

#22 drobbyb

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Posted 25 October 2009 - 12:46 AM

I am sorry to find that we have yet another product that is not working, at least not the sample you got.



I was really hoping that there would be some sign that it works by now. I have to agree with OldMan47, this one too looks like it just dosen't work.

Mature media is still the best option.

#23 backtotropical

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Posted 25 October 2009 - 04:52 PM

I must admit I'm very disappointed to hear that it isn't working. I've been waiting a long time to find someone who is testing this stuff.

Timothy Hovanec wrote 3 pioneering scientific papers on nitrifying bacteria in aquariums in his quest to produce what we know now as Bio-Spira.

Unfortunately, Dr Hovanec left Marineland (who manufacture Bio-Spira) and reports suggest that Bio-Spira no longer works like it used to.

Hovanec owns the patent on aquarium bacteria solutions containing Nitrospira (the bacteria responsible for nitrite oxidation in aquariums, previously thought to be Nitrobacter before Hovanec's research), so I assume that he isn't allowing Marineland to use "his" formula any longer.

Is the new one a refrigerated product?

#24 rebrn

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Posted 26 October 2009 - 08:53 PM

Hello everyone, In answer to the question is this a refridgerated product, the answer is yes and no. It states that it does not need to be refridgerated it can be kept at room temperature, but if at room temp shelf life is 6 months, if regridgerated then 1 year shelf life.

So I was very dissapointed in the product so I decided to contact Dr. Tim and let him know ... I was able to find an email address via his website. Anyway, I expected to get a form letter providing me with proper instructions etc. However, to my suprise, Dr. Tim him self emailed me back. This is what he told me:

1. His product reccomends an ammonia concentration of 2ppm, if you have a geater ammonia level you need proponiately more product. ie - ammonia of 4ppm requres double the product.

2. I used a water conditioner that contained an aldehyde - this can effect his product, although as my water sat for 2 weeks prior to adding his product he did not feel that was the primary issue.

So what to do, as I still had product left, he suggested using the remaining product in the tanks (bringing my total dosed amount to 3.5 ml/gallon in each tank). Low and behold 12 hours after adding additional product sucess! The5 gallon tank still showed no nitrItes but had a nitrAte reading of 5ppm. The 12 gallon also showed no nitrItes but had a trace amount of nitrAtes (definately not orange put a slight orange tinge to the test).

So perhaps it did not work as quickly as I did not fully following the directions. He said that the amount I used would still work with ammonia of 4ppm it would just take longer. The instructions state best results with ammonia of 2ppm and no higer then 5ppm.

I will update the first post with the results after adding more product ... at work right now and don't have the log with me.

Thanks

#25 waterdrop

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Posted 26 October 2009 - 10:22 PM

Well thanks rebrn, continues to be interesting! :)

~~waterdrop~~

#26 rebrn

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Posted 27 October 2009 - 02:06 AM

Ok I have caught the first post up with the test results. Now I know what some of you are thinking, because I am thinking it too. That the nitrAtes seen could have arrived as a result of the natural cycling process and adding additional doseage of bacteria product could have just been a coincidence. I do agree with this thought and remain skeptical of the product thus far, however, I do find it courious that I had essentially no activity at all in the tank untill I added the additional dosing, and it would appear that I have skipped the nitrIte spikes and gone straight to nitrAte. So while I remain skeptical, I am now hopeful that this product is actually working.

Oh and sorry I mis spoke in the post above, it was 12 hours when I saw a trace nitrAtes and 24 hours before I saw definate 5ppm nitrAtes in the 5 Gallon. The 12 Gallon was 24 hours before trace - have not seen definate nitrAtes yet but I think I am being overly cautious about what I call orange and just really dark yellow :blush:

Edited by rebrn, 27 October 2009 - 02:20 AM.


#27 waterdrop

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Posted 27 October 2009 - 10:39 AM

I suppose its possible the reason Dr. Hovanec's mentioning 2ppm is because he's adding in the fact that the majority of the organic material in the bottle (my guess here) will just go out into the tank and add a dose of material that the heterotrophs can munch on, creating still more ammonia, thus potentially raising the concentration above 5ppm and encouraging the wrong species of autotroph.

Dr. Hovanec himself, I believe, is the one who taught us about the alternate species coming in around 8ppm concentration and blocking out the Nitrospira (& possibly the Nitrosomonas, not sure) that we want. So, if I'm remembering that correctly and I'm pretty sure I am, I would expect him to be one of the ones concious of not taking the concentration above 5ppm.

~~waterdrop~~

#28 OldMan47

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Posted 27 October 2009 - 10:07 PM

I am sure it was Dr Tim who gave us that tidbit. He is also the one who observed that excessive use of dechlorinators, more than a double dose, would greatly slow the bacterial development.

#29 rebrn

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Posted 28 October 2009 - 10:03 PM

It was Dr. Tim, that info is actually on his website in one of the papers that he wrote.

#30 rebrn

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Posted 04 November 2009 - 05:33 AM

hello everyone, sorry I haven't added the updates in a while, I was having problems with my internet. At anyrate, I have caught up the testing results in the first post. The 5 gallon is coming along quite nicely, actually moving fairly quickly now :good: . The 12 gallon is still taking its time ... but today got a definate nitrate reading of 5 which is great because if it follows the 5 gallon it should start to move quickly now. At any rate, I think I can safely say that the product didn't necessarily hurt anything but I don't feel it speed up the process at all. I could be wrong in that, I am afterall an impatient person, so perhaps I was expecting too much from the product (I was kinda hoping for the miracle overnight cycle product :blush: ). But I suppose it did help somewhat (espically after adding the second dose) in the 5 gallon tank but not really that much and certainly hardly at all in the 12 gallon tank.

I suppose the final verdict is try it if you want it didn't seem to hurt anything, the cost isn't that much, about $10 for 2 oz. Just remember if you do decide to try it, be sure that your ammonia is around 2ppm (definately not above 5ppm), and if your ammonia is above 2ppm make sure to add double the dose, and also be sure that your dechlorinator does not contain an aldehyde procuct. If you follow those guidelines it may help to speed your cycle a little bit, but don't expect miracles. :)




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